响应面法优化丁香叶总皂苷提取工艺

采用响应面分析法对丁香叶总皂苷提取工艺进行优化。以总皂苷提取率为考察指标,在单因素实验基础上,采用响应面分析法对乙醇浓度、液料比、提取温度和提取时间进行优化。结果表明,乙醇回流提取总皂苷的最佳工艺条件为:乙醇浓度82%、液料比25∶1 m L/g、提取温度85℃、提取时间116 min、提取次数2次,在此条件下总皂苷提取率为(69.655±0.0145)mg/g,与理论值仅相差0.461 mg/g,表明此模型准确可靠,是提取丁香叶总皂苷的可行方法。 

Optimization of extraction process of total saponins from Syringa oblate Lindl. leaves by response surface methodology

Total saponins extraction from Syringa oblate Lindl. leaves was performed using the ethanol reflux extraction.Response surface methodology( RSM),based on a three level,four variable Box- Behnken design( BBD),was employed to obtain the best possible combination of ethanol concentration,liqued /material ratio,extraction temperature and extraction time. The optimum extraction conditions were as follows: ethanol concentration of 82%,liqued / material ratio of 25∶ 1 m L / g,extraction temperature of 85 ℃,extraction time of 116 min and extraction times of 2.Under these conditions,the experimental yield was( 69.655 ± 0.0145) mg / g.The difference of extract rate between actual value and theoretical value was 0.461 mg / g,therefore the extraction process obtained by response surface methodology was stable and reasonable,accurate and reliable. It was a feasible method to extract the total saponins from Syringa oblate Lindl.leaves.