超声破碎辅助蜗牛酶提取杏鲍菇蛋白工艺优化

为显著提高杏鲍菇子实体蛋白提取率，采用超声波破碎辅助蜗牛酶水解充分破碎菌体细胞壁。综合应用响应面、正交设计与人工神经网络模型相结合的试验设计方法，分别对超声破碎处理和蜗牛酶水解条件进行了优化，同时与直接热水碱提蛋白法进行了比较。结果表明，超声处理条件为超声功率300 W、超声时间26 min、水料比1.95∶5（mL/g），在此条件下破碎效果最好，提取率达到了48.82%；最佳蜗牛酶水解条件为温度42.8 ℃、pH 4.8、酶用量7%、时间1.5 h，在该条件下蛋白提取率为75.92%，相较热水碱提法提高了33.35%，结果表明利用超声辅助蜗牛酶水解杏鲍菇细胞壁能显著提高蛋白提取率。

Optimization of Ultrasonic-Assisted Snailase Hydrolysis for Extraction of Protein from Pleurotus eryngii

The aim of this work was to enhance the efficiency of protein extraction from fruit bodies of Pleurotus eryngii by cell disruption using ultrasonic irradiation followed by snailase hydrolysis. Response surface methodology, orthogonal array design and artificial neural network model were used in combination to optimize the conditions for ultrasonic irradiation and subsequent enzymatic hydrolysis, respectively. The protein extraction efficiencies of the presented method and the hot alkali method were compared. The best efficiency of cell disruption was achieved upon ultrasonic treatment for 26 min at 300 W power with a solid-to-water ratio of 1.95:5 (mL/g), which resulted in a protein yield of 48.82%. The optimum hydrolysis conditions that provided maximum protein yield of 75.92% were determined as 42.8 ℃, 4.8, 7% and 1.5 h for temperature, pH, enzyme dosage and hydrolysis time, respectively, which was increased by 33.35% compared with hot alkali extraction. These results showed that ultrasonic-assisted snailase hydrolysis could significantly increase the efficiency of protein extraction from P. eryngii fruit bodies.