| 微波辅助碱—酶法提取酵母β-1,3-葡聚糖工艺优化 |
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| 引用本文： | 杨锡洪,高学丽,解万翠,李子琪. 微波辅助碱—酶法提取酵母β-1,3-葡聚糖工艺优化[J]. 食品与机械, 2016, 32(2): 145-148 |
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| 作者姓名： | 杨锡洪 高学丽 解万翠 李子琪 |
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| 作者单位： | 青岛科技大学化学与分子工程学院，山东 青岛 266042；广东海洋大学食品科技学院，广东 湛江 524088;广东海洋大学食品科技学院，广东 湛江 524088；青岛科技大学化工学院，山东 青岛 266042 |
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| 基金项目： | 湛江市科技计划项目资助（编号：2013A05006）；2015年国家级大学生创新创业训练计划项目（编号：201510426027） |
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| 摘 要： | 为建立酵母β-1,3-葡聚糖高效、快速的提取方法,以细胞壁为原料,在传统碱—酶法基础上,辅助微波加热,采用响应面法优化提取工艺,通过二次回归模型分析得出最佳工艺为:微波功率420 W,加热时间5min,酶添加量2 550U/g。该条件下β-1,3-葡聚糖总糖含量预测值为87.44%,验证值为87.94%。
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| 关 键 词： | 酵母；β-1 3-葡聚糖；微波辅助；碱—酶法 |
Optimization on beta-1,3-glucan from yeast by |
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| YANGXihong,GAOXueli,XIEWancui,LIZiqi. Optimization on beta-1,3-glucan from yeast by[J]. Food and Machinery, 2016, 32(2): 145-148 |
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| Authors: | YANGXihong GAOXueli XIEWancui LIZiqi |
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| Affiliation: | College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China; College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China |
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| Abstract: | For finding a rapid and environmental friendly way to extract zymosan (beta-1,3-glucan), a method was established by microwave-assisted heating at low alkaline concentration, which was using the cell wall as a raw material and based on traditional enzyme alkaline. Extraction process was optimized by using response surface method, the optimum process conditions for quadratic regression model analysis were as followed: microwave power 420 W, heating time 5 min; the quantity of enzyme 2 550 U/g. Under this condition, the total sugar value could reach 87.44%, and the verificatio n results was 87.94%. |
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| Keywords: | yeast beta-1, 3-glucan microwave assisted alkaline enzyme method |
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