蜂花粉中黄酮类化合物的鉴定和含量测定

以24种不同蜂花粉为材料,对其中微量特征成分黄酮类化合物进行鉴定和归纳。建立一种高压液相色谱-串联三重四级杆质谱法快速测定蜂花粉中8种黄酮类化合物的方法。破壁的试样经乙醇溶液提取后加酸水解,经反相色谱分离,质谱定性并测定8种黄酮类化合物含量,外标法定量。该方法线性良好,R2均大于0.999,检出限为0.15³.0 mg/kg,精密度为3.2%⁶.6%,回收率在91.2%¹00.2%,此方法操作简便,抗基质干扰、结果可靠。分析结果证实,蜂花粉中黄酮类化合物通常有槲皮素、山奈酚、芦丁等。可为识别蜂花粉的植物来源,鉴别真伪和建立相应的指纹图谱提供研究方法和实验依据。 

Identification and detection of flavonoids in bee pollen

To establish a quick,accurate and sensitive approach to analyse flavonoid contents using 24 kinds of bee pollen as material,a method called ultra- high pressure liquid chromatograph with tandem triple level 4 pole mass spectrometry had been set up in this research,aimed to detect 8 types of flavone.After crushed the wall of samples by temperature,differential method stepped by the acid hydrolysis through ethanol extraction and finally diluted with methanol solution,the targets aimed to detect were seperated by reversed- phase chromatography,measured by mass spectrometry and quantitated by external standard method. Statistics indicated that this method had a good linear with R2> 0.999,its RSD was less than 6.6 percent and more than 3.2 percent. The LOD was between0.15 and 3.0 mg / kg along with a hight recovery rate ranged from 91.2% to 100.2%. Depended on its easier operation,reliable result with lower matrix interference,this method could be applied to other flavonoids contents in bee pollen such as quercetin,kaempferol,rutin and so on.Last but not the least,it could also provide experimental bases and research methodology to identify the plant origin for bee pollen,distinguish authenticity further to build revelant fingerprint.