Illumina Miseq平台深度测定酸奶中微生物多样性

目的:建立Illumina Miseq深度测序筛查酸奶中微生物的方法,分析酸奶中微生物的多样性。方法:以9种酸奶样品为研究对象,提取酸奶中细菌基因组DNA,应用高通量测序技术测定细菌的16S r DNA v1-v3变异区序列,得到9种样品中微生物群体分布和丰度、以及不同样品间的菌种差异与进化关系。结果:酸奶中微生物主要为厚壁菌门（Firmicutes）,占据99.6%之高,其中厚壁菌门主要以链球菌属（Streptococcus、87.1%）、乳杆菌属（Lactobacillus、10.3%）、乳球菌属（Lactococcus、0.3%）组成,9种酸奶中有3种同时含有链球菌属和乳杆菌属,其余6种样品中链球菌属几乎占据全部（>97%）。结论:Illumina Miseq深度测序技术可快速精确深入掌握酸奶中微生物多样性,结果对比显示不同酸奶菌种同质化程度高,其中链球菌属占绝对优势,同时发现部分酸奶标签标示不符。 

Microbial diversity of yogurt depth detected by Illumina Miseq platform

Objective: Establishing a method based on Illumina Miseq deep sequencing to analyze the microbial diversity of yogurt. Methods: Took 9 kinds of yogurt samples as the research objects and extracted the whole genome of bacteria of yogurt. The v1- v3 variation region of 16 S r DNA were detected by high throughput sequencing technology to obtain the distribution and abundance of microbial community in 9 samples as well as the species difference and evolution relationship of bacteria among these samples.Results: The results showed that the main microbes of the yogurt were Firmicutes( 99.6%) which mainly composed by Streptococcus( 87.1%),Lactobacillus( 10.3%),and Lactococcus( 0.3%).There were 3 kinds of yogurt containing Streptococcus and Lactobacillus with a high number,Streptococcus( 97%) accounted for almost all in the other six samples.Conclusion: Using Illumina Miseq deep sequencing technology can master the microbial diversity of yogurt quickly and accurately. The results showed that the bacteria of yogurt were highly similar and streptococcus took a dominant position.At the same time,it was found that some yogurt labels didn't match the analysis results well.