卵转铁蛋白对树突状细胞功能成熟的影响

目的：探究卵转铁蛋白（ovotransferrin，OVT）对树突状细胞（dendritic cells，DCs）功能成熟的影响。方法：通过混合淋巴反应检测OVT对DCs刺激T淋巴细胞增殖能力的影响，酶联免疫吸附分析（enzyme-linkedimmunosorbent assay，ELISA）检测DCs上清液中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白介素12p70（interleukin-12p70，IL-12p70）和IL-10细胞因子含量，Western blotting检测胞外信号调节激酶（extracellular signalregulated kinase，ERK）、p38丝裂原活化蛋白激酶（p38 mitogen activated protein kinase，p38 MAPK）和c-Jun氨基末端激酶（c-Jun N-terminal kinase，JNK）信号蛋白表达量。结果：OVT可以提高DCs刺激T淋巴细胞增殖的能力，并诱导DCs分泌TNF-α以及IL-12p70，且呈一定的剂量依赖关系。OVT可能是通过激活JNK和p38 MAPK信号通路以及抑制ERK信号通路上调细胞因子TNF-α、IL-12p70表达，从而促进DCs成熟。结论：OVT能促进DCs功能成熟，对机体的免疫具有调节作用。

Effect of Ovotransferrin on Functional Maturation of Dendritic Cells

This study was designed to investigate the effect of ovotransferrin (OVT) on functional maturation of dendritic cells (DCs). The capacity of DCs for inducing the proliferation of allogeneic spleen T lymphocytes was measured by mixed lymphocyte reaction and the production of tumor necrosis factor-α (TNF-α), interleukin-12p70 (IL-12p70) and interleukin-10 (IL-10) in supernatants from DCs was determined by enzyme-linked immunosorbent assay (ELISA), and the activation of c-Jun N-terminal kinase (JNK), extracellular signal regulated kinase (ERK), and p38 mitogen activated protein kinase (p38 MAPK) were detected by Western blotting. The results showed that OVT promoted the ability of DCs to induce the proliferation of allogeneic spleen T lymphocytes and increased the production of TNF-α and IL-12p70 in a dose-dependent manner. Up-regulation of JNK and p38 MAPK activation and blockage of ERK activation by OVT might contribute to the expression of TNF-α and IL-12p70, so that OVT could effectively regulate DC maturation. These results indicate that ovotransferrin can induce functional maturation of DCs and can be a potential functional factor to regulate body immune.