| 羊肚菌多糖抑制人乳腺癌细胞MDA-MB-231增殖和诱导细胞凋亡研究 |
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| 引用本文： | 李 谣,陈金龙,王丽颖,张月巧,吴素蕊,明 建.羊肚菌多糖抑制人乳腺癌细胞MDA-MB-231增殖和诱导细胞凋亡研究[J].食品科学,2016,37(21):214. |
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| 作者姓名： | 李 谣 陈金龙 王丽颖 张月巧 吴素蕊 明 建 |
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| 作者单位： | 1.西南大学食品科学学院，重庆 400715；2.中华全国供销合作总社昆明食用菌研究所，云南 昆明 650223；
3.西南大学 国家食品科学与工程实验教学中心，重庆 400715 |
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| 摘 要： | 以黑脉羊肚菌多糖为原料，研究羊肚菌多糖对人乳腺癌细胞MDA-MB-231（以下简称MDA）增殖和凋亡的影响。结果表明：在无细胞毒性范围内，羊肚菌多糖能显著抑制人乳腺癌细胞MDA的增殖，半数有效浓度（median effective concentration，EC50）值为0.096 mg/mL，同时人乳腺癌细胞MDA表现出多种细胞凋亡的形态学变化。免疫印迹检测实验结果显示，羊肚菌多糖能抑制B淋巴细胞瘤-2（B-cell lymphoma-2，Bcl-2）蛋白表达，促进Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）表达，同时增加Bax/Bcl-2蛋白表达比值，并呈现出剂量依赖效应。说明羊肚菌多糖能抑制人乳腺癌细胞MDA增殖，促进细胞凋亡。
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| 关 键 词： | 羊肚菌 多糖 人乳腺癌细胞MDA-MB-231 细胞增殖 细胞凋亡 |
Polysaccharides from Morchella angusticeps Peck Inhibit Cell Proliferation and Induce Apoptosis in MDA-MB-231 Human Breast Cancer Cells |
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| LI Yao,CHEN Jinlong,WANG Liying,ZHANG Yueqiao,WU Surui,MING Jian.Polysaccharides from Morchella angusticeps Peck Inhibit Cell Proliferation and Induce Apoptosis in MDA-MB-231 Human Breast Cancer Cells[J].Food Science,2016,37(21):214. |
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| Authors: | LI Yao CHEN Jinlong WANG Liying ZHANG Yueqiao WU Surui MING Jian |
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| Affiliation: | 1. College of Food Science, Southwest University, Chongqing 400715, China;
2. Kunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooporatives, Kunming 650223, China;
3. National Food Science and Engineering Experimental Teaching Center, Southwest University, Chongqing 400715, China |
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| Abstract: | The effects of polysaccharides from Morchella angusticeps Peck (PMEP) on the proliferation and apoptosis of
MDA-MB-231 human breast cancer cells were investigated in this study. The results showed that PMEP could significantly
inhibit the proliferation of MDA-MB-231 in the non-cytotoxic concentration range with a median effective concentration
(EC50) of 0.096 mg/mL. MDA-MB-231 cells also showed a series of morphological changes associated with apoptosis.
Western blotting assay showed that PMEP could inhibit the expression of B-cell lymphoma-2 (Bcl-2) protein, increase the
expression of Bcl-2 associated X protein (Bax) and Bax/Bcl-2 ratio in a dose-dependent manner. In conclusion, PMEP could
inhibit the proliferation and induce the apoptosis of MDA-MB-231 cells. |
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| Keywords: | Morchella esculenta polysaccharide MDA-MB-231 human breast cancer cell cell proliferation cell apoptosis |
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