甜玉米芯多糖提取及抗凝血活性初探

对甜玉米芯多糖进行提取并纯化,并对其抗凝血活性进行初步研究。采用水提法提取甜玉米芯多糖,三氯乙酸、酶及Sevag-酶法脱除蛋白,双氧水和树脂法进行脱色研究,多糖醇沉分级后进行体内外抗凝血研究。结果表明:水提法提取甜玉米芯多糖得率为16.7%,Sevag-酶法脱蛋白效果最好,蛋白脱除率为84.94%,多糖保留率为70.74%。D301R阴离子大孔树脂脱色效果最佳,脱色率为46.17%,多糖保留率为99.23%。甜玉米芯醇沉分级后多糖对出血时间（BT）和凝血时间（CT）均有显著影响（p<0.05）,各组分均可以延长活化部分凝血活酶时间（APTT）和凝血酶时间（TT）（p<0.05）,但对凝血酶原时间（PT）影响不显著（p>0.05）,其中SCP-80抗凝血效果较好。 

Extraction and anticoagulant activities of polysaccharides from sweet corncob

The methods of extraction,purification and anticoagulant activities of polysaccharides were researched from sweet corncob. The polysaccharides were extracted with the method of water,the removing protein of polysaccharides with the methods of trichloroacetic acid,enzyme and Sevag-enzyme respectively. The decoloration of polysaccharides by hydrogen peroxide and resin,anticoagulant activities of polysaccharides were researched after grading use alcohol. The results showed that the optimum yield of polysaccharides was up to16.7% with the method of water.The deproteinization effect of sevag-enzyme was best,the removal rate of protein and the recovery rate of polysaccharides of sweet corn cobs was 84.94% and 70.74%,respectively. The decolorization effect of D301 R resin was best,the decolorization rate and the recovery rate of polysaccharides of sweet corn cobs was 46.17% and 99.23%,respectively. Anticoagulant activities in vivo and vitro showed that polysaccharides and different fractions of sweet corncobs had a significant influence on bleeding time and blood coagulation time( p < 0.05),all the components can prolong APTT and TT( p < 0.05),but had no significant effect on PT( p > 0.05),the anticoagulant activities of SCP-80 was better.