油松花粉多酚抗氧化性研究

通过超声波辅助乙醇浸提法提取油松花粉多酚,采用AB-8大孔吸附树脂分离纯化油松花粉多酚,以V_C为阳性对照,分别采用Fe³⁺-还原法、DPPH·清除法、水杨酸比色法和邻苯三酚自氧化法来研究油松花粉多酚的还原能力及其对DPPH·、·OH、O₂^-·的清除作用,实验结果显示,当浓度均为6μg/m L时,油松花粉多酚对DPPH·清除率比V_C高出16.33%,显著高于V_C（p<0.05）;当浓度均为8μg/m L时,油松花粉多酚对·OH清除率比V_C高出32.03%,显著高于V_C（p<0.05）;当浓度均为6μg/m L时,油松花粉多酚对O₂^-·清除率比V_C高出10.53%,高于V_C但不显著;当浓度均为10μg/m L时,油松花粉多酚在700 nm处的吸光度为0.712,显著高于V_C(A_{700 nm}=0.353667,p<0.05),说明油松花粉多酚还原力强于V_C。实验数据表明油松花粉多酚具有较强的还原能力和清除氧自由基的作用,且其还原能力及清除率均高于V_C。结果表明油松花粉多酚具有显著的抗氧化活性。 

Antioxidant activities of polyphenols from pinus tabulaeformis pollen

The pinus tabulaeformis pollen polyphenols were extracted by ultrasonic with ethanol,and they were separated and purified by AB- 8 macroporous adsorption resin,the positive comparison was V_C ,the reducing power of pinus tabulaeformis pollen polyphenols and their scavenging effect on DPPH·,·OH,O₂^-·were researched by Fe³⁺- reducing method,DPPH·reducing method,salicylic acid colorimetry and pyrogallol autoxidation method.The experimental results showed that when the concentrations both were 6 μg / m L,pinus tabulaeformis pollen polyphenols was 16.33% significant higher than that of V_C on DPPH·clearance( p < 0.05),when the concentrations both were 8 μg / m L,pinus tabulaeformis pollen polyphenols was 32.03% significant higher than that of V_C on ·OH clearance( p < 0.05),when the concentrations both were 6 μg / m L,pinus tabulaeformis pollen polyphenols was10.53% unsignificant higher than that of V_C on O₂^-· clearance.When the concentrations both were 10 μg /m L,the absorbance of pinus tabulaeformis pollen polyphenols was 0.712 at 700 nm,was significantly higher than that of V_C ( A₍700 nm)= 0.353667,p < 0.05),so the reducing power of pinus tabulaeformis pollen polyphenols was higher than that of V_C . The results showed that pinus tabulaeformis pollen polyphenols had stronger reducing power and scavenging effectt on oxygen free radicals,and its clearance rate was higher than that of V_C . In conclusion,the pinus pollen polyphenols have significant antioxidant activity.