枯草芽孢杆菌发酵牦牛血制备抗氧化肽工艺优化

为优化枯草芽孢杆菌液态发酵制备牦牛血抗氧化肽工艺,以酵解液的羟基自由基(·OH)清除率为主要指标,研究发酵时间、接种量、底物浓度对酵解液抗氧化效果的影响,在该基础上进行响应面优化试验。确定最佳发酵条件为:底物浓度75g/L,接种量2.5%(V/V),发酵时间69.5h。在该条件下制备出·OH清除率为74.48%的牦牛血抗氧化肽,·OH清除率理论值为75.78%,最终发酵上清液多肽含量为2.31mg/mL。

Optimization of fermentation conditions for preparation of antioxidant peptides

In order to optimize fermentation conditions for production preparation of Yak blood antioxidant peptides with Bacillus subtilis, ·OH scavenging rate was taken as index to analyze the effects of fermentation time, inoculum concentration, substrate concentration on the antioxidant result with response surface methodology. The optimum fermentation conditions were determined as followsfollowed: 75 g/L of substrate concentration, 69.5 h of fermentation time, 2.5%(V/V) of inoculum concentration. Under such conditions, the ·OH scavenging rate was 74.48%, which was close to the theoretical value of 75.77%. Eventually, the content of peptides in fermented supernatant was 2.31 mg/mL.