响应面优化纤维素酶法提取绣球菌多糖

为了提高得率,本文采用纤维素酶提取绣球菌多糖。通过单因素实验考察了酶添加量、酶解温度、酶解时间、液料比、p H对多糖得率的影响。在此基础上,选取对多糖得率影响较大的因素进行响应面优化分析。最优工艺为:酶添加量为1.3%、酶解温度为40℃、酶解时间2.5 h、液料比42 m L/g、p H为5.5,在此条件下多糖得率为22.90%±0.26%。 

Optimization of cellulase extraction of Sparassis crispa polysaccharides using response surface methodology

In order to get higher extraction efficiency,the polysaccharides from Sparassis crispa were extracted using cellulase.A single- factor study was designed to investigate the effects of different factors on the yield of polysarccharides,such as enzymatic addition,enzymatic hydrolysis temperature,enzymatic hydrolysis time,liquid /material ratio and p H value.Based on the results of single- factor assay,several factors were chosen to be further optimized by response surface methodology. The optimal process parameters were showd as follows: enzymatic addition of 1.3%,extraction temperature of 40 ℃,hydrolysis time of 2.5 hours,the liquid / solid ratio of 42 m L / g,and p H5.5.Under these above conditions,the content of polysaccharides could be up to 22.90% ± 0.26%.