红花籽油对D-半乳糖致衰老小鼠模型的抗衰老作用

为了研究红花籽油的抗衰老作用,采用红花籽油灌胃D-半乳糖致衰老小鼠模型,利用试剂盒测定这些小鼠脑肝组织的丙二醛(MDA)含量,单胺氧化酶(MAO)、谷胱甘肽过氧化酶(GSH-Px)及超氧化物歧化酶(SOD)活性。统计分析各组之间与衰老和抗衰老有关成分含量的差异显著性。结果表明:红花籽油组小鼠脑肝组织MDA含量和MAO活性显著(P0.05)或极显著(P0.01)低于衰老模型组;GSHPx、总超氧化物歧化酶(T-SOD)、铜锌超氧化物歧化酶(CuZn-SOD)和锰超氧化物歧化酶(Mn-SOD)活性极显著高于衰老模型组。红花籽油的抗衰老机制可能是:通过清除自由基,抑制自由基诱导的脂质过氧化反应,减少脂质过氧化物产生;通过改善机体新陈代谢,促进D-半乳糖分解和代谢,改善物质能量代谢,调节与衰老有关的基因表达,抑制MAO基因表达,降低脑肝组织的MAO含量;促进GSHPx、CuZn-SOD和Mn-SOD基因表达,提高脑肝组织GSHPx、CuZn-SOD和Mn-SOD含量。红花籽油能显著降低衰老模型小鼠脑肝组织MDA含量和MAO活性,显著提高GSH-Px、SOD-1和SOD-2活性,具有显著的抗老作用。

Study on anti-aging effect of safflower oil on the mouse model aged by D-galactose

To study the anti-aging functions of safflower oil, mice model aged by D-galactose were administered safflower oil by oral gavage. The content of malondialdehyde (MDA) and the activity levels of monoamine oxidase (MAO), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in the brain and liver of these mice were determined with biochemical kits, respectively. The significance of the differences in the content of the components associated with aging and anti-aging among each group was analyzed statistically. The main results were as follows. The MDA content and the activity level of MAO in the brain and liver of mice in the groups administered safflower oil were significantly lower (P<0.05) or very significantly lower (P<0.01) than those of the aging model group. However, the activity levels of GSH-Px, total SOD (T-SOD), CuZn-SOD, and Mn-SOD in the brain and liver of the mice were very significantly higher than those of the aging model group. It was supposed that the anti-aging mechanism of Safflower seed oil might be it could inhibit the lipid peroxidation induced by free radicals and reduces lipid peroxide by scavenging free radicals. By improving body''s metabolism, it promoted the decomposition and metabolism of D-galactose, and improved the material energy metabolism, and then regulated the expression of genes related to aging. Meanwhile, the expression of MAO gene was inhibited to reduce MAO content in liver and brain, which resulted in promoting the expression of the genes, including GSH-Px, CuZn-SOD, and Mn-SOD. This could increase the content of GSH-Ps, CuZn-SOD, and Mn-SOD both in liver and brain. We concluded that the safflower seed oil could significantly reduce content of MDA and MAO and increase that of GSH-Ps, CuZn-SOD, and Mn-SOD in liver and brain of aging mice models, performing the obvious anti-aging function.