不同贮藏时期朝鲜族大酱致突变及抗突变作用

为探究朝鲜族大酱的致突变与抗突变作用随贮藏时间延长的变化,在改良的Ames试验测定其致突变活性的基础上,结合小鼠骨髓细胞微核试验对不同贮藏时期的朝鲜族大酱的抗突变活性进行了测定。结果显示:大酱提取物致突变性呈阴性;而抗突变性随贮藏时间的延长及浓度的增加而逐渐增强,对MNNG及Trp-p-1诱导TA100及TA98菌株的抗突变作用均在贮藏第12月时达到最大值,并存在剂量—浓度—效应关系。同时小鼠骨髓细胞微核试验结果表明贮藏12个月的大酱样品微核数最低,微核抑制率最高达46.5%。该试验研究结果显示贮藏12个月的朝鲜族大酱抗突变作用最强且无致突变性,可为朝鲜族大酱进一步向营养、功能方向发展,及今后朝鲜族大酱的基础研究和癌症饮食疗法的推广提供试验依据。

Study on mutagenic and anti-mutafenic effect of Korean soybean paste with different storage period

In order to explore the mutagenic and anti-mutagenic effect on the different storage periods of Korean soybean paste. In this study, on the basis of detecting mutagenicity effect by modified Ames test, and detecting the anti-mutagenic effects of the Korean soybean paste samples with different storage time have on Mouse bone marrow micronucleus test. Result: the mutagenicity of Korean soybean paste were negative; Anti-mutagenic effect were enhanced with the extension of storage time and the concentration, the anti-mutagenic effect of TA100 and TA98 strains induced by Trp-p-1 and MNNG reached the maximum value at 12 months of storage. And there was a dose-concentration-response relationship. At the same time, there was significant inhibitory effect of Korean soybean paste extracts with storage 12 months on mouse bone marrow micronucleus, the highest inhibition rate was up to 46.5% in the test. The results showed the strongest anti-mutagenic effect of Korean soybean paste was which stored for 12 months and no mutagenicity found, provided an experimental proof for the further development of Korean soybean paste in nutritional, functional, and for basic research and the promotion of cancer diet.