红树莓不同溶剂提取物抗氧化活性

以红树莓鲜果为原料,采用体外抗氧化方法研究不同溶剂提取对红树莓抗氧化活性的影响。分别以水、25%乙醇、50%乙醇、75%乙醇、100%乙醇、乙酸乙酯、正丁醇为提取溶剂,测定提取物中总酚、黄酮、原花青素和花色苷含量;研究提取物体外抗氧化活性的差异;对活性物质含量与抗氧化能力进行相关性分析。结果表明,50%乙醇粗提物的总酚、黄酮、原花青素和花色苷含量均最高,分别为257.19、10.48、406.45、22.47 mg/100 g;7种不同红树莓提取物中50%乙醇提取物的总还原力与V_C相近,50%和75%乙醇提取物对DPPH·清除能力强于V_C,7种溶剂提取物对羟基自由基清除能力均弱于V_C;红树莓7种溶剂提取物中总酚与对DPPH·的清除率及总还原能力的相关性较大。比较发现,50%~75%的乙醇水溶液能够更好的溶出红树莓中的活性成分,而具有更强的抗氧化活性。 

Antioxidant activity of different solvents extracts from red raspberry

The antioxidant capacities of seven different extractions of red raspberry,which were extracted with distilled water,25% ethanol,50% ethanol,75% ethanol,100% ethanol,ethyl acetate and n- butyl alcohol,respectively,were compared using antioxidant determining methods in vitro. Results showed that the highest contents of total phenols,flavonoids,proanthocyanidins and anthocyanins were determined in 50% ethanol extracts,which were respectively up to 257.19,10.48,406.45 mg /100 g and 22.47 mg /100 g.The total reducing capacities of 50% ethanol extracts approached to Vc's.Except for 50% and 75% ethanol extracts,the others held weaker capacity of scavenging DPPH·than that of Vc's and none held stronger capacity of scavenging OH·than that of Vc's.The analysis pointed that in extractions from red raspberry,there were relatively significant correlations between the total phenols and the capacities of scavenging DPPH·and the total reducing capacities,respectively.Conclusively,50% ~75% ethanol- aqueous solvent of red raspberry extracts held stronger antioxidant activity.