| 响应面法优化乙醇/硫酸铵双水相体系萃取洋葱黄酮的研究 |
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| 引用本文： | 肖连冬,王莹,于海彦,李慧星,程爽.响应面法优化乙醇/硫酸铵双水相体系萃取洋葱黄酮的研究[J].中国食品添加剂,2019(8):80-85. |
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| 作者姓名： | 肖连冬 王莹 于海彦 李慧星 程爽 |
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| 作者单位： | 1.南阳理工学院生物与化学工程学院;2.河南省工业微生物资源与发酵技术重点实验室 |
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| 摘 要： | 以洋葱为原料,以黄酮提取率为主要指标,对乙醇/硫酸铵双水相体系萃取洋葱黄酮进行研究。通过单因素试验,考察了乙醇质量分数、硫酸铵质量分数、洋葱粉加量对双水相萃取洋葱黄酮分配情况及提取率的影响。并采用Box-Behnken中心组合试验和响应面分析,对洋葱黄酮提取条件进行优化。试验结果表明,乙醇/硫酸铵双水相体系萃取洋葱黄酮的最佳条件为:乙醇质量分数32.1%,硫酸铵质量分数20.4%,洋葱粉加量0.45g,在此条件下,洋葱黄酮的提取率达3.74%。
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| 关 键 词： | 双水相体系 洋葱 黄酮 响应面法 |
Response surface optimization for extraction of onion flavone by ethanol/ammonium sulfate aqueous two-phase system |
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| XIAO Lian-dong,WANG Ying,YU Hai-yan,LI Hui-xing,CHENG Shuang.Response surface optimization for extraction of onion flavone by ethanol/ammonium sulfate aqueous two-phase system[J].China Food Additives,2019(8):80-85. |
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| Authors: | XIAO Lian-dong WANG Ying YU Hai-yan LI Hui-xing CHENG Shuang |
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| Affiliation: | (School of Biological and Chemical Engineering,Nanyang Institute of Technology,Nanyang 473000;Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology,Nanyang 473000) |
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| Abstract: | XIAO Lian-dong;WANG Ying;YU Hai-yan;LI Hui-xing;CHENG Shuang(School of Biological and Chemical Engineering,Nanyang Institute of Technology,Nanyang 473000;Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology,Nanyang 473000) |
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| Keywords: | aqueous two-phase system onion flavone response surface methodology |
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