基质固相分散萃取-气相色谱-串联质谱法同时测定蔬菜中195 种农药残留

目的：建立一种蔬菜中195 种农药的气相色谱-质谱检测方法。方法：样品用乙酸乙酯匀浆提取后，经基质固相分散萃取法净化，气相色谱-质谱仪的选择反应监测模式-进行测定，内标法定量。结果：在0.01～1.00 mg/L质量浓度范围内，所有农药的线性相关系数均大于0.99，方法的定量限范围为1.9～25.5 μg/kg。分别在菜心、生菜、黄瓜、芹菜、土豆等蔬菜中进行3 个添加水平（10、20、100 μg/kg）实验，平均回收率为37.6%～136.7%，相对标准偏差为0.2%～15.3%。结论：该方法快速、灵敏、准确、高通量，具有良好的回收率和稳定性，较好地解决了样品本底成分复杂带来的基质干扰，适用于蔬菜中195 种农药残留量的快速测定。

Simultaneous Determination of 195 Pesticide Residues in Vegetables Using Solid Phase Dispersive xtraction-Gas Chromatography-Tandem Mass Spectrometry

Objective: To develop a method for the determination of 195 pesticide residues in vegetables using gas
chromatography with tandem mass spectrometry (GC-MS-MS) under a selected reaction monitoring (SRM) mode. Methods:
The target compounds were extracted from samples with ethyl acetate and the extract was cleaned up by off-line solid phase
dispersive extraction technique. The analytes were quantified by an internal standard method. Results: In the linear range
(0.01–1.00 mg/L), the correlation coefficient for each pesticide was higher than 0.99. The average recoveries at three spiked
levels (10, 20 and 100 μg/kg) varied from 37.6% to 136.7% with relative standard deviations (RSDs) between 0.2% and 15.3%.
The limits of quantification (LOQ) (signal/noise ratio = 10) were 1.9–25.5 μg/kg. Conclusion: The method is rapid, sensitive,
accurate and high throughput, without matrix interference and suitable for the analysis of 195 pesticide residues in vegetables.