陈皮中川陈皮素的提取分离及改性

以陈皮中川陈皮素的提取、分离纯化以及改性为目的，采用无水乙醇浸提陈皮得浸膏，以氯仿为萃取剂萃取浸膏，得到多甲氧基黄酮混合物，再以硅胶为吸附剂、乙酸乙酯和石油醚的混合液为洗脱剂，采用柱色谱法分离多甲氧基黄酮化合物，经高效液相色谱和气相色谱-质谱检测并与标准品对比，最终确证得到川陈皮素纯品。研究从川陈皮素到5-去甲基川陈皮素的合成路线，优化了改性条件，在浓盐酸与无水乙醇体积比为1∶9条件下，将川陈皮素加热回流反应24 h，可以得到纯度大于97%的5-去甲基川陈皮素。

Extraction and Modification of Nobiletin from Tangerine Peel

The aim of this research is to extract, purify and modify nobiletin from dried orange peel. The extraction process
was carried out by means of infiltration. The extract was further extracted with chloroform to obtain a fraction rich in
polymethoxylated flavonoids and the target compounds were separated on a silica gel column with a mobile phase consisting
of a mixture of ethyl acetate and petroleum ether. Pure nobiletin was obtained as indicated by reversed phase HPLC and GC-MS
and through comparison with reference standards. The conversion conditions from nobiletin to 5-demethylnobiletin was optimized
and it was found that 5-demethylnobiletin with a purity higher than 97% was produced when the reaction was performed for 24 h
under heat refluxing condition with a volume ratio of 1:9 between concentrated hydrochloric acid and absolute ethanol