The molecular mechanism of membrane proteins probed by evanescent infrared waves |
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Authors: | Nyquist Rebecca M Ataka Kenichi Heberle Joachim |
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Affiliation: | Forschungszentrum Jülich, IBI-2: Structural Biology, 52425 Jülich, Germany. |
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Abstract: | The catalytic action of membrane proteins is vital to many cellular processes. Yet the molecular mechanisms remain poorly understood. We describe here the technique of evanescent infrared difference spectroscopy as a tool to decipher the structural changes associated with the enzymatic action of membrane proteins. Functional changes as minute as the protonation state of individual amino acid side chains can be observed and linked to interactions with a ligand, agonist, effector, or redox partner. |
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Keywords: | attenuated total reflection spectroscopy bacteriorhodopsin cytochrome c oxidase IR spectroscopy membrane proteins reaction mechanisms |
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