| 利用DH群体构建烤烟分子标记遗传连锁图 |
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| 引用本文： | 肖炳光,徐照丽,陈学军,申爱荣,李永平,朱军.利用DH群体构建烤烟分子标记遗传连锁图[J].中国烟草学报,2006,12(4):35-40. |
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| 作者姓名： | 肖炳光 徐照丽 陈学军 申爱荣 李永平 朱军 |
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| 作者单位： | 1.中国烟草育种研究(南方)中心, 云南省烟草科学研究所 玉溪 653100; |
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| 基金项目： | 国家烟草专卖局资助项目;云南省烟草专卖局资助项目 |
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| 摘 要： | 以烤烟品种SpeightG-28和NC2326杂交得到的137个DH系为作图群体材料,通过ISSR和RAPD标记的遗传连锁分析,构建了包括27个连锁群、由10个ISSR标记和147个RAPD标记组成的烤烟分子标记遗传连锁图。该图谱覆盖长度1838.2cM,平均图距14.1cM。连锁群上有22个分子标记表现偏分离,其中10个集中在连锁群LG9上,其余分散在不同连锁群。这是国内外构建的第一张烤烟分子标记遗传连锁图,为烤烟性状的基因定位及分子标记辅助选择奠定了良好基础。
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| 关 键 词： | 烤烟 ISSR RAPD 遗传连锁图 |
| 文章编号： | 1004-5708(2006)04-0035-06 |
| 收稿时间： | 03 22 2006 12:00AM |
| 修稿时间： | 2006年3月22日 |
Genetic linkage map constructed by using a DH population for the flue-cured tobacco |
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| XIAO Bing-guang,XU Zhao-li,CHEN Xue-jun,SHEN Ai-rong,LI Yong-ping,ZHU Jun.Genetic linkage map constructed by using a DH population for the flue-cured tobacco[J].Acta Tabacaria Sinica,2006,12(4):35-40. |
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| Authors: | XIAO Bing-guang XU Zhao-li CHEN Xue-jun SHEN Ai-rong LI Yong-ping ZHU Jun |
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| Affiliation: | 1.China Tobacco Breeding(Southern) Center, Yunnan Institute of Tobacco Science, Yuxi 653100;2.College of Plant Protection, Yunnan Agricultural University, Kunming 6502013;3.Institute of Bioinformatics, Zhejiang University, Hangzhou 310029 |
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| Abstract: | A molecular genetic map of flue-cured tobacco was constructed with a 137 doubled haploid (DH) population from a cross of 2 flue-cured tobacco varieties Speight G-28 and NC2326 by using ISSR and RAPD as markers. The map consisted of 27 linkage groups which included 10 ISSR and 147 RAPD markers, and covered 1838.2 cM with an average distance of 14.1 cM. Among the 22 distorted markers distributed in the map, 10 markers were mapped on the linkage group LG9, and the others were mapped on diverse linkage groups respectively. It is the first molecular genetic linkage map of flue-cured tobacco in the world and is fundamental to the genetic mapping of important traits and marker-assisted selection for flue-cured tobacco. |
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| Keywords: | ISSR RAPD |
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