Fermentative hydrogen production by a new alkaliphilic Clostridium sp. (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay,New Caledonia |
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Authors: | Nan Mei Nesrine Zergane Anne Postec Gael Erauso Angélique Ollier Claude Payri Bernard Pelletier Marie-Laure Fardeau Bernard Ollivier Marianne Quéméneur |
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Affiliation: | 1. Aix Marseille Université, CNRS, Université de Toulon, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille, France;2. Institut pour la Recherche et le Développement (IRD), Centre de Nouméa, MIO UM 110, Promenade Laroque, 98848 Nouméa, New Caledonia |
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Abstract: | The hydrogen-producing strain PROH2 pertaining to the genus Clostridium was successfully isolated from a shallow submarine hydrothermal chimney (Prony Bay, New Caledonia) driven by serpentinization processes. Cell biomass and hydrogen production performances during fermentation by strain PROH2 were studied in a series of batch experiments under various conditions of pH, temperature, NaCl and glucose concentrations. The highest hydrogen yield, 2.71 mol H2/mol glucose, was observed at initial pH 9.5, 37 °C, and glucose concentration 2 g/L, and was comparable to that reported for neutrophilic clostridial species. Hydrogen production by strain PROH2 reached the maximum production rate (0.55 mM-H2/h) at the late exponential phase. Yeast extract was required for growth of strain PROH2 and improved significantly its hydrogen production performances. The isolate could utilize various energy sources including cellobiose, galactose, glucose, maltose, sucrose and trehalose to produce hydrogen. The pattern of end-products of metabolism was also affected by the type of energy sources and culture conditions used. These results indicate that Clostridium sp. strain PROH2 is a good candidate for producing hydrogen under alkaline and mesothermic conditions. |
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Keywords: | Biohydrogen production Clostridium Hydrothermal system Alkaliphilic Serpentinization hydA genes |
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