Enhancement in hydrogen production by co-cultures of Bacillus and Enterobacter |
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| Authors: | Sanjay K.S. Patel Prasun Kumar Sanjeet Mehariya Hemant J. Purohit Jung-Kul Lee Vipin C. Kalia |
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| Affiliation: | 1. Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India;2. Department of Biotechnology, University of Pune, Pune 411007, India;3. Environmental Genomics Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur 440020, India;4. Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea |
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| Abstract: | Defined co-cultures of hydrogen (H2) producers belonging to Citrobacter, Enterobacter, Klebsiella and Bacillus were used for enhancing the efficiency of biological H2 production. Out of 11 co-cultures consisting of 2–4 strains, two co-cultures composed of Bacillus cereus EGU43, Enterobacter cloacae HPC123, and Klebsiella sp. HPC793 resulted in H2 yield up to 3.0 mol mol−1 of glucose. Up-scaling of the reactor by 16-fold resulted in a corresponding increase in H2 production with an actual evolution of 7.44 L of H2. It constituted 58.2% of the total biogas. Continuous culture evolution of H2 by co-cultures (B. cereus EGU43 and E. cloacae HPC123) immobilized on ligno-cellulosic materials resulted in 6.4-fold improvement in H2 yield compared to free floating bacteria. This synergistic influence of B. cereus and E. cloacae can offer a better strategy for H2 production than undefined or mixed cultures. |
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| Keywords: | Bacillus Dark fermentation Enterobacter Hydrogen Klebsiella |
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