Impact of voltage and pulse delivery mode on the efficacy of pulsed light for the inactivation of Listeria |
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| Affiliation: | 1. College of Food Science and Technology, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan 430070, China;2. Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China;3. College of agriculture and life sciences, Cornell University, Ithaca, New York, United States;1. Department of Food Technology and Bioprocess Engineering, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany;2. Institute for Micro Process Engineering, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany;1. Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria;2. OFI — Austrian Research Institute for Chemistry and Technology, Brehmstrasse 14 A, 1110 Vienna, Austria |
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| Abstract: | Listeria innocua inactivation by pulsed light (PL) was evaluated at different settings and voltages, to establish the best treatment conditions and post-treatment handling for further implementation of PL in the food industry. Fluences up to 0.2 J/cm2 were applied to superficially inoculated TSA agar plates (4.5–5 log cfu/cm2). Inactivation was calculated, and log-linear and Weibull models were applied. A fluence of 0.2 J/cm2 applied in a single pulse inactivated 3.8 log cfu/cm2, while sequential application of this fluence yielded an inactivation between 1.5 and 2.5 log cfu/cm2 depending on the delivery mode (consecutive flashing or with 5 min-holding times under ambient light or in the dark). Data from consecutive PL treatment were fitted with the Weibull model. No photoreactivation following PL was observed after 120-min exposure to ambient light in any of the conditions assayed. This study showed that flashing with a single pulse at higher voltage would offer the highest inactivation of Listeria.Industrial relevanceThis study offered information of practical interest to establish pulsed light processing and post-processing conditions for the control of Listeria spp. in the food industry, for instance in ready-to-eat (RTE) products. The use of higher voltages provided higher inactivation and allowed to minimise the number of flashes. If sequential treatments are to be applied, the treatment is more effective if short holding times are kept between pulses. The post-processing illumination conditions do not influence the efficacy of PL treatment. |
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