A promising innovative technique for mycotoxin detoxification from beverages using biofilms of lactic acid bacteria |
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| Affiliation: | 1. Research Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadat Campus, Beirut, Lebanon;2. Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadat Campus, Beirut, Lebanon;3. Centre d''Analyses et de Recherche (CAR), Unité de Recherche Technologies et Valorisation agro-Alimentaire (UR-TVA), Faculté des Sciences, Université Saint-Joseph de Beyrouth, Campus des sciences et technologies, Mar Roukos, Matn, Lebanon;1. Dairy Engineering Section, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560030, India;2. Economics and Statistics Section, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560030, India;3. Livestock Research Centre, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560030, India;4. Dairy Technology Section, Southern Regional Station, ICAR-National Dairy Research Institute, Bengaluru, Karnataka 560030, India;1. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy;2. Department of Industrial Engineering, Alma Mater Studiorum—University of Bologna, Bologna, Italy;3. Interdepartmental Centre for Industrial Research in Health Sciences and Technologies, University of Bologna, Bologna, Italy;4. Alma Plasma srl Bologna, Italy;5. Department of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, Italy;6. Inter-Departmental Centre for Agri-Food Industrial Research, University of Bologna, via Quinto Bucci 336, 47521 Cesena (FC), Italy;1. Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand;2. School of Food and Advanced Technology, Massey University, Private Bag 11 222, Palmerston North, New Zealand;3. Fonterra Research and Development Centre, Palmerston North, New Zealand;4. School of Agricultural Technology, Walailak University, Nakhon Sri Thammarat 80160, Thailand |
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| Abstract: | This study assessed the removal of aflatoxin M1 (AFM1) and ochratoxin A (OTA) from artificially contaminated whole UHT milk and red grape juice, respectively, using biofilms from Lactobacillus rhamnosus GG. Using ELISA, the level of AFM1 and OTA removal from beverages was determined depending on various factors. Biofilms of various ages demonstrated varying degrees of AFM1 removal capacity from phosphate-buffered saline (PBS). Different levels of AFM1 contaminated whole UHT milk (0.1, 0.2, and 1 μg/L) and OTA contaminated red grape juice (2 and 4 μg/L) were tested in the detoxification process. The binding ability of mycotoxins was improved by increasing the biofilm surface area up to 70 cm2. L. rhamnosus GG biofilm was effective in removing mycotoxins within a short contact time ranging from 1 to 10 min. The proportion of bound AFM1 and OTA by L. rhamnosus GG biofilm was 64.6 and 98.3% respectively. A new machine has been proposed and used as a trial for detoxication purposes which would be a promising application in liquid food industries. |
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