Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures |
| |
Authors: | Takashi Kobayashi Takemasa Matsuo Yukitaka Kimura Shuji Adachi |
| |
Affiliation: | (1) Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku Kyoto, 606-8502, Japan |
| |
Abstract: | The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80–100 °C to measure the residual activity as
a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30–60 wt% water contents.
The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation
for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution. |
| |
Keywords: | Candida antarctica fraction B lipase Gaussian distribution Glycerol Immobilization Thermal inactivation |
本文献已被 SpringerLink 等数据库收录! |