| 超高效液相色谱-串联质谱多组分检测牛奶中外源性激素残留 |
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| 引用本文： | 张爱芝,王全林. 超高效液相色谱-串联质谱多组分检测牛奶中外源性激素残留[J]. 食品科学, 2010, 31(6): 208-212. DOI: 10.7506/spkx1002-6630-201006049 |
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| 作者姓名： | 张爱芝 王全林 |
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| 作者单位： | 宁波市产品质量监督检验研究院 |
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| 基金项目： | 浙江省质检系统科技计划项目(20070106) |
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| 摘 要： | 在多反应监测模式条件下采用正离子采集模式建立牛奶中7 种激素的超高效液相色谱- 质谱/ 质谱(UPLCMS/MS)检测方法。样品以甲醇溶液为提取剂超声辅助提取，经LC-C18 柱净化，经BEH-C18(100mm × 2.1mm，1.7μm)柱分离后进行UPLC-MS/MS 多反应监测模式下的定性及定量分析。7 种激素方法检出限为0.01～0.25μg/kg，定量限为0.06～0.5μg/kg，添加水平为10μg/kg 时，平均回收率为83%～124%，相对标准偏差为4.3%～24%。该法灵敏度高、检出限低、分析时间短、操作简便，可应用于实际样品检测。
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| 关 键 词： | 超高效液相色谱- 质谱/ 质谱 性激素 牛奶 |
| 收稿时间： | 2009-03-20 |
Simultaneous Determination of Exogenous Anabolic Steroid Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry(UPLC-MS/MS) |
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| ZHANG Ai-zhi,WANG Quan-lin. Simultaneous Determination of Exogenous Anabolic Steroid Residues in Milk by Ultra Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry(UPLC-MS/MS)[J]. Food Science, 2010, 31(6): 208-212. DOI: 10.7506/spkx1002-6630-201006049 |
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| Authors: | ZHANG Ai-zhi WANG Quan-lin |
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| Affiliation: | Ningbo Academy of Product Quality Supervision and Inspection, Ningbo 315041, China |
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| Abstract: | A rapid,specific and highly sensitive method was developed for the determination of 7 exogenous anabolic steroid residues in milk based on ultra performance liquid chromatography-electrospray tandem mass spectrometry(UPLC-MS/MS) in the positive ion mode using multiple reaction monitoring(MRM).Samples were extracted with methanol with the assistance of ultrasonic and cleaned up using LC-C18 solid phase extraction cartridge.Qualitative and quantitative analysis was carried out in the MRM mode after chromatogr... |
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| Keywords: | ultra performance liquid chromatography-electrospray tandem mass spectrometry(UPLC-MS/MS) anabolic steroid milk |
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