Recovery of Giardia cysts from water: Centrifugation vs filtration |
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| Authors: | Bob Holman Floyd Frost Byron Plan Karen Fukutaki Walter Jakubowski |
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| Affiliation: | 1. Health Services Division, Department of Social and Health Services, Seattle, WA, U.S.A.;2. Health Effects Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH, U.S.A. |
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| Abstract: | Methods for the recovery of Giardia cysts from large volume (1900 l.) water samples were experimentally examined in the laboratory and applied to 22 surface water supplies around Washington state. Orlon yarn-wound filters (7 and 1 μm porosity) were compared, with higher cyst recovery rates observed for 1 μm filters. For each experiment, half the recovered material was processed through a series of screens while the other half was processed through an algae (Foerst) centrifuge. Both methods recovered cysts from 1900 l. water samples containing as few as 6000 cysts, with higher recovery rates observed for the algae centrifuge. Cysts were recovered more often from surface water sources implicated in outbreaks (3 of 4) than from sources not implicated in known outbreaks (2 of 18). |
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| Keywords: | Address correspondence to: Floyd Frost Public Health Laboratory 1409 Smith Tower Seattle WA 98104 U S A |
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