| Sabin株脊髓灰质炎灭活疫苗纯化工艺的建立 |
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| 引用本文： | 衡燮,高娜,伍胤杰,林小瑞,周武刚,王微,唐聪,姜述德,杨净思,孙明波.Sabin株脊髓灰质炎灭活疫苗纯化工艺的建立[J].粉末涂料与涂装,2010,23(4). |
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| 作者姓名： | 衡燮 高娜 伍胤杰 林小瑞 周武刚 王微 唐聪 姜述德 杨净思 孙明波 |
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| 作者单位： | 中国医学科学院,北京协和医学院,医学生物学研究所,昆明,650118 |
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| 基金项目： | 国家"重大新药创制"科技重大专项项目，云南省科技创新强省计划项目 |
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| 摘 要： | 目的建立Sabin株脊髓灰质炎灭活疫苗(IPV)纯化工艺。方法采用Sepharose CL-6B凝胶过滤和DEAE-Sepharose Fast Flow离子交换层析两步纯化法纯化脊髓灰质炎病毒,进行各项检定后再经除菌过滤和灭活。结果Ⅰ、Ⅱ、Ⅲ型病毒纯化后的蛋白去除率均不低于98.81%,病毒滴度分别为8.75、8.50和8.63lgCCID50/ml,DNA残留量均<100pg/ml。除菌过滤和灭活后,D抗原含量略有下降。结论已建立了Sabin株IPV的纯化工艺,纯化后制备出了高纯度的Sabin株IPV。
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| 关 键 词： | 脊髓灰质炎灭活疫苗 凝胶过滤层析 离子交换层析 |
Development of Purification Procedure of Inactivated Poliomyelitis Vaccine Prepared with Sabin Strain |
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| HENG Xie,GAO Na,WU Yin-jie,et al.Development of Purification Procedure of Inactivated Poliomyelitis Vaccine Prepared with Sabin Strain[J].Chinese Journal of Biologicals,2010,23(4). |
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| Authors: | HENG Xie GAO Na WU Yin-jie |
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| Affiliation: | HENG Xie,GAO Na,WU Yin-jie,et al(Institute of Medical Biology,Chinese Academy of Medical Sciences , Peking Union Medical College,Kunming 650118,China) |
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| Abstract: | Objective To develop a purification procedure of inactivated poliomyelitis vaccine(IPV)prepared with Sabin strain. Methods Poliomyelitis virus was purified by Sepharose CL-6B gel filtration and DEAE-Sepharose Fast Flow ion exchange chromatography, subjected to control tests then sterilized by filtration and inactivated. Results All the removal rates of protein in poliomyelitis virus typesⅠ, Ⅱ and Ⅲ were not less than 98.81%, and the residual DNA contents were less than 100 pg / ml, while the virus titers were 8. 75, 8. 50 and 8. 63 lg CCID50 / ml respectively. The D antigen content in the virus after sterilization by filtration and inactivation decreased slightly. Conclusion A purification procedure of IPV prepared with Sabin strain was developed, and IPV with high purity was prepared. |
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| Keywords: | Inactivated poliomyelitis vaccine(IPV) Gel filtration chromatography Ion exchange chromatography |
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