Amplification of Bcl I region of the factor VIII gene by PCR |
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Authors: | R Ishak LC Khim |
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Affiliation: | Hematology Division, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia. |
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Abstract: | A study was initiated to amplify by polymerase chain reaction (PCR), a short factor VIII gene fragment containing the Bcl I restriction site from hemophilia patients using published primer sequences. Preliminary findings indicated that the resulting fragment is 142 bp long. This fragment, when digested with Bcl I restriction enzyme produced two fragments, 99 bp and 43 bp in length. Polymorphism in the Bcl I region can be used to detect carrier state in the family members of the hemophiliacs. |
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