| 牛链球菌L-(+)-乳酸脱氢酶基因的克隆及在大肠杆菌中表达 |
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| 引用本文： | 张黎,韦宇拓,黄彦,杜丽琴,黄日波.牛链球菌L-(+)-乳酸脱氢酶基因的克隆及在大肠杆菌中表达[J].食品与发酵工业,2005,31(9):24-28. |
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| 作者姓名： | 张黎 韦宇拓 黄彦 杜丽琴 黄日波 |
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| 作者单位： | 广西大学生命科学与技术学院发酵与酶工程研究所,南宁,530005 |
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| 摘 要： | 利用PCR方法从牛链球菌(Streptococcus bovis)基因组DNA中扩增出了L(+)乳酸脱氢酶基因(ldh),并将其连接到表达载体pSE380上,得到重组质粒pSEldh,将重组质粒转化到大肠杆菌菌株JM109。利用SDS-PAGE分析ldh表达产物的分子量为36KD,重组菌株的乳酸脱氢酶酶活力为168.2U/mL,最适反应温度25℃,最适作用pH为5.0,重组质粒的稳定性达99.9%。
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| 关 键 词： | L-乳酸 L-(+)-乳酸脱氢酶 克隆 表达 牛链球菌 |
| 收稿时间： | 04 8 2005 12:00AM |
| 修稿时间： | 06 7 2005 12:00AM |
Cloning and Expression of the Gene Encoding the L- ( + )Lactate Dehydrogenase from Streptococcus bovis in Escherichia coli |
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| Zhang Li,Wei Yutuo,Huang Yan,Du Liqin,Huang Ribo.Cloning and Expression of the Gene Encoding the L- ( + )Lactate Dehydrogenase from Streptococcus bovis in Escherichia coli[J].Food and Fermentation Industries,2005,31(9):24-28. |
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| Authors: | Zhang Li Wei Yutuo Huang Yan Du Liqin Huang Ribo |
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| Affiliation: | Institute of Fermentation and Enzyme Engineering, Guangxi University, Nanning, 530005, China |
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| Abstract: | The gene ldh encoding L-(+)-lactate dehydrogenase was amplified from genome DNA of Streptococcus bovis by PCR.The recombinant plasmid pSE-ldh was constructed by inserting ldh into expression vector pSE380 and then transformed into E.coli JM109. SDS-PAGE showed that the relative molecular weight of the expression product was about 36 kD.The activity of L-(+) lactate dehydrogenase of recombinant strain was 168.2 U/mL. The optimal temperature was 25℃and the optimal pH was 5.0. The stability of the plasmid pSE-ldh in E.coli JM109 was 99.9%. |
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| Keywords: | L- lactate L-(+)lactate dehydrogenase cloning expression Streptococcus bovis |
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