| 荧光光谱法测定啤酒中核黄素的含量 |
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| 引用本文： | 周丽丽,胡北,崔胜云. 荧光光谱法测定啤酒中核黄素的含量[J]. 延边大学学报(自然科学版), 2012, 0(1): 78-82 |
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| 作者姓名： | 周丽丽 胡北 崔胜云 |
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| 作者单位： | 延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 |
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| 摘 要： | 探讨了酸度和光降解作用对核黄素吸收和荧光光谱的影响.利用改进后的荧光光谱法测定了不同品牌啤酒样品中游离核黄素的含量,结果表明,所选啤酒样品中的核黄素含量在0.025 97～0.034 29μg/mL范围内.该方法操作简单、结果可靠,为定量测定基体复杂样品中的核黄素含量提供了新的方法.
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| 关 键 词： | 核黄素 荧光光谱法 紫外可见光谱法 荧光猝灭 |
Riboflavin analysis in beer using fluorescence spectrometry |
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| ZHOU Li-li,HU Bei,CUI Sheng-yun. Riboflavin analysis in beer using fluorescence spectrometry[J]. Journal of Yanbian University (Natural Science), 2012, 0(1): 78-82 |
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| Authors: | ZHOU Li-li HU Bei CUI Sheng-yun |
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| Affiliation: | (Key Laboratory of Natural Resources of the Changbai Mountain ＆ Functional Molecules(Yanbian University),Ministry of Education,Yanji 133002,China) |
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| Abstract: | Uv-vis spectrometric and fluorometric properties of riboflavin are investigated in different acidity and light degradation.A modified fluorescence method is used to detect the content of riboflavin in different brands of beer.The results indicated that the content of riboflavin in beers ranges from 0.025 97-0.034 29 μg/mL.The method effectively eliminates metrix interferences and applicable to determination complex matrix samples. |
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| Keywords: | riboflavin fluorescence spectrometry Uv-vis spectrometry fluorescence quenching |
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