响应曲面法优化酶法提取银杏叶总黄酮

本文研究了酶法提取银杏叶总黄酮的工艺。在单因子试验结果的基础上,采用响应曲面法(responsesur-facemethodology,RSM)研究了酶浓度、pH值、酶解温度和酶解时间对银杏叶总黄酮提取得率的影响。结果表明,纤维素酶具有较好的酶解能力,当酶浓度为100U/ml,酶解时间为116min,温度为40℃,pH4.5时,总黄酮得率达到2.63%,相对单一醇提法得率提高了约30%。

Optimization of Total Flavonoids Extraction from Ginkgo biloba Leaves by Enzymatic Hydrolysis Using Response Surface Methodology

The technology of total flavonoids extracted from Ginkgo biloba leaves by enzymatic hydrolysis was studied. Based on single factor test, the effects of the concentration of enzyme, pH value, temperature and time of enzymatic hydrolysis on the extraction yield of total flavonoids from Ginkgo biloba leaves were stuied by response surface methodology (RSM). The results indicated that cellulase shows the better ability of enzymatic hydrolysis. The optimum conditions of enzymatic hydrolysis are: :100U/ml concentration, 116min time, 40℃ and 4.5 pH value. The highest yield of total flavonoids was 2.63%. Comparing with single extraction with alcohol, the total flavonoids yield increase by 30%.