Identification of vasopressor phospholipid in crude soybean lecithin |
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Authors: | Akira Tokumura Kenji Fukuzawa Yasue Akamatsu Sadaji Yamada Tetsuya Suzuki Hiroaki Tsukatani |
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Affiliation: | (1) Faculty of Pharmaceutical Sciences, Tokushima University, Shomachi, 770 Tokushima, Japan;(2) Present address: Research Institute for Food Science, Kyoto University, Gokasho, Uji, 611 Kyoto, Japan |
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Abstract: | The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH-20. It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography-mass spectrometry analysis of TMS-deacylated product and acetolysis product. Nuclear magnetic resonance analysis favored the 1-monoacyl isomer over the 2-isomer. By enzymic determination with L-3-glycerophosphate dehydrogenase, the isolated phospholipid was identified as 1-monoacyl-L-3-glycerophosphate. Gas chromatographic examination revealed that it was composed of a large percentage of unsaturated fatty acids, especially linoleic acid. The activity of isolated lysophosphatidic acid was slightly less than that of synthetic 1-linoleoyl-L-3-glycerophosphate. |
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