| 葛根素及总黄酮含量测定 |
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| 引用本文： | 赵颖,王然. 葛根素及总黄酮含量测定[J]. 饮料工业, 2012, 15(1): 37-40 |
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| 作者姓名： | 赵颖 王然 |
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| 作者单位： | 1. 武汉天河机场动力能源公司动力能源保障部,湖北武汉,432202
2. 湖北工业大学生物工程学院,湖北武汉,430068 |
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| 摘 要： | 采用HPLC法和UV法检测了4种植物饮料中葛根素和总黄酮的含量。结果表明,葛根植物饮料中葛根素和总黄酮含量均最高,分别为0.135mg/ml和0.263mg/ml;竹叶植物饮料次之,葛根素和总黄酮含量分别为0.108mg/ml和0.228mg/ml;罗汉果植物饮料中葛根素和总黄酮含量均为最低,分别为0.032mg/ml和0.084mg/ml。HPLC法和UV法平均回收率分别为100.01%和100.03%;RSD分别为0.12%和0.25%,方法准确、稳定,重现性高。
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| 关 键 词： | 植物饮料 葛根素 总黄酮 高效液相色谱法 紫外分光光度法 |
Determination of puerarin and total flavonoids |
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| ZHAO ying , WANG Ran. Determination of puerarin and total flavonoids[J]. Beverage Industry, 2012, 15(1): 37-40 |
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| Authors: | ZHAO ying WANG Ran |
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| Affiliation: | 1 Power and Energy Security Division,Power and Energy Company of Wuhan Tianhe Airport,Wuhan 432202,Hubei,China; 2 Bioengineering College,Hubei University of Technology,Wuhan 430068,Hubei,China) |
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| Abstract: | Puerarin and total flavonoids in 4 categories of vegetable beverages were determined by the HPLC and UV methods.The results showed that puerarin and total flavonoids were both highest in the Pueraria vegetable beverages（0.135 and 0.263 mg/ml,respectively）;the bamboo vegetable beverages were next to them,with the contents of puerarin and total flavonoids being 0.108 and 0.228 mg/ml,respectively;puerarin and total flavonoids were lowest in the Mangosteen vegetable beverages,0.032 and 0.084 mg/ml,respectively.As for the HPLC and UV methods,the average recoveries were 100.01% and 100.03%,and the RSD 0.12% and 0.25%,respectively.They were both accurate and stable,with high reproducibility. |
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| Keywords: | vegetable beverage puerarin total flavonoids HPLC UV |
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