| 用活性碳分离纯化苯丙氨酸与酪氨酸的研究 |
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| 引用本文： | 陈瑞峰,陈璐,陈廷登. 用活性碳分离纯化苯丙氨酸与酪氨酸的研究[J]. 浙江工业大学学报, 2006, 34(4): 373-376 |
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| 作者姓名： | 陈瑞峰 陈璐 陈廷登 |
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| 作者单位： | 1. 浙江工业大学,生物与环境工程学院,浙江,杭州,310032
2. 浙江工业大学,之江学院,浙江,杭州,310024 |
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| 摘 要： | 根据活性炭对L-苯丙氨酸与L-酪氨酸的选择性吸附特性，通过活性炭吸附及氨水、乙醇溶液解吸，从啤酒酵母提取液中成功地分离提取了符合标准的L一苯丙氨酸与L-酪氨酸；实验研究了苯丙氨酸与酪氨酸在不同pH值溶液中在活性炭上的吸附平衡，确定了以pH值5．8上柱，1mol氨水和60％乙醇二步洗脱分离提取两种氨基酸的工艺，为从啤酒酵母中提取氨基酸提供了一条有效的途径。
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| 关 键 词： | 活性炭 L-苯丙氨酸 L-酪氨酸 吸附 解吸 |
| 文章编号： | 1006-4303（2006）04-0373-04 |
| 收稿时间： | 2005-03-25 |
| 修稿时间： | 2005-03-25 |
Study on isolation and purification of L-Phenylalanin and L-Tyrosine by active carbon |
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| CHEN Rui-feng,CHEN Lu,CHEN Ting-deng. Study on isolation and purification of L-Phenylalanin and L-Tyrosine by active carbon[J]. Journal of Zhejiang University of Technology, 2006, 34(4): 373-376 |
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| Authors: | CHEN Rui-feng CHEN Lu CHEN Ting-deng |
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| Affiliation: | 1. College of Biological and Environmental Engineering Zhejiang University of Techrology, Hangzhou 310032, China 2. Zhijiang College, Zhejiang University of Technology, Hangzhou 310024, China |
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| Abstract: | L-Phenylalanin(L-Phe) and L-Tyrosine(L-Tyr) were separated from the beer yeast extract using their selective adsorption on active carbon followed by elution with aqueous ammonia and ethanol.The obtained substances are in accordance with their corresponding product standards.The adsorption of L-Tyr and L-Phe on commercial activated carbon at different pH values in the solution has been investigated.An optimal separation technology is proposed with the adsorption carried out at the pH value of 5.8 followed by elution with 1mol/L aqueous ammonia and 60% ethanol,respectively.The method gives an effective separation of amino acids from the beer yeast extract. |
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| Keywords: | active carbon L-Phenylalanine L-Tyrosine adsorption elute |
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