| 罗丹明6G荧光特性及其在荧光猝灭法中的应用 |
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| 引用本文： | 张瑞华,;崔建升,;孟素英.罗丹明6G荧光特性及其在荧光猝灭法中的应用[J].河北机电学院学报,2014(3):252-261. |
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| 作者姓名： | 张瑞华 ;崔建升 ;孟素英 |
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| 作者单位： | [1]河北科技大学环境科学与工程学院,河北石家庄050018; [2]河北省污染防治生物技术实验室,河北石家庄050018 |
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| 摘 要： | 罗丹明类荧光染料具有摩尔吸光系数高、光稳定性好、对pH值不敏感,较宽的波长范围和较高的量子产率等优点。详细分析了罗丹明6G这种荧光染料的荧光特性,并按荧光共振能量转移、形成杂多酸离子缔合物和与碘化钾反应生成离子缔合物3种不同的荧光猝灭方式,综述了罗丹明6G在荧光猝灭法中的广泛应用。
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| 关 键 词： | 罗丹明6G 荧光特性 荧光猝灭 应用 |
Fluorescence characteristics of rhodamine 6G and its application in fluorescence quenching method |
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| Affiliation: | ZHANG Ruihua, CUI Jiansheng, MENG Suying (1. School of Environmental Science and Engineering, Hehei University of Science and Technology, Shijiazhuang Hebei 050018, China;2. Pollution Prevention Biotechnology Laboratory of Hebei Province, Shijiazhuang Hebei 050018, China) |
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| Abstract: | Rhodamine fluorescent dyes has a high molar extinction coefficient, light stability, pH-insensitivity, wide wave- length range and a high quantum yield, etc.. This article analyzes the fluorescence characteristics of the fluorescent dye rhoda- mine 6G detailedly. By fluorescence resonance energy transfer, the formation of ion-association complex heteropoly acid and the ion-association complex generating reacted with potassium iodide, the three different fluorescence quenching methods prove the wide use of rhodamine 6G in the fluorescence quenching method. |
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| Keywords: | rhodamine 6G fluorescence characteristics fluorescence quenching application |
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