Cell viability of Listeria monocytogenes biofilms |
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| Affiliation: | 1. University of Florida, Department of Food Science and Human Nutrition, P.O. Box 110370, Gainesville, FL 32611-0370, USA;2. University of Florida, Plant Pathology Department, P.O. Box 110680, Gainesville, FL 32611-0680, USA;3. Food and Drug Administration, HFS-700, 5100 Paint Branch Parkway, College Park, MD 20740, USA;4. Land O''Lakes, Inc, 4001 Lexington Avenue North, Arden Hills, MN 55126, USA;5. Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore;1. Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland;2. Institut Pasteur, Microbial Evolutionary Genomics, Paris, France;3. CNRS, UMR3525, Paris, 75015, France;4. University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France;1. Institute of Agro-Food Standard and Testing Technology, Shanghai Academy of Agricultural Science, No. 1000 Jinqi Road, Shanghai, 201403, People''s Republic of China;2. Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA, 19038, United states;1. Food, Nutrition and Health, Faculty of Land and Food Systems, The University of British Columbia, 2205 East Mall, Vancouver, BC V6T 1Z4, Canada;2. Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Nowoursynowska 159 C, 02-776 Warsaw, Poland;3. Agriculture and Agri-Food Canada, Summerland Research and Development Centre, 4200 Highway 97 South, Summerland, British Columbia V0H 1Z0, Canada |
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| Abstract: | Several authors have reported biofilm formation by Listeria monocytogenes, and it is suspected that biofilms form a unique niche for extended survival of this foodborne pathogen in food-processing environments. We have evaluated growth of two L. monocytogenes strains (Murray and 7148) in biofilms and analysed the relationship between culturable and viable-but-non-culturable (VBNC) cells. Biofilms were grown on glass slides in static conditions at 37°C for up to 10 days. Culturable cells for L. monocytogenes Murray grew to 105cfu cm−2within 2 days, while L. monocytogenes 7148 required 4 days to reach these cell numbers. After 2 days, cell counts of L. monocytogenes Murray decreased, followed by another increase with cell numbers reaching almost 106cfu cm−2on day 10. In contrast, cell counts of L. monocytogenes 7148 stayed close to 105cfu cm−2until day 10. VBNC cells of L. monocytogenes Murray increased with biofilm age while this was not seen for strain 7148. Also, swabbing removed biofilms of strain Murray more easily than strain 7148. Comparisons of viable counts obtained for swabbed and in situ biofilms indicated that these strain differences are due either to variable composition of extracellular polymeric substances in the two biofilms or to different cell physiology of the two strains. |
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