| 浓香型白酒窖泥中细菌多样性的免培养技术分析 |
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| 引用本文： | 汤斌,刘金英,周庆武,李安军,万春环,汤有宏.浓香型白酒窖泥中细菌多样性的免培养技术分析[J].食品与发酵工业,2012(7):43-47. |
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| 作者姓名： | 汤斌 刘金英 周庆武 李安军 万春环 汤有宏 |
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| 作者单位： | 安徽工程大学微生物发酵安徽省工程技术研究中心;安徽古井贡酒股份有限公司 |
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| 基金项目： | 安徽省自然科学基金(No.KJ2007A018) |
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| 摘 要： | 采用免培养(culture independent)技术直接从浓香型白酒窖泥中提取细菌微生物混合基因组DNA(也叫元基因组DNA),利用细菌16S rDNA通用引物扩增窖泥细菌的序列,根据16S rDNA序列对细菌多样性进行初步分析。采用PCR扩增技术、分子克隆技术以及序列同源性分析等方法测定细菌的16S rDNA,通过与基因数据库中相似菌群序列同源性的比较,得到样品菌种多样性,分别构建系统发育树。结果显示,细菌含有几大类群,表现出高度的细菌多样性。共分为Uncultured bacterium、Clostridium、Lactobacillus、Eubacterium和Syntroph-omonas五个细菌分类。
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| 关 键 词： | 浓香型白酒 窖泥 微生物多样性 免培养法 系统发育树 |
Phylogenetic Diversity Analysis of Bacteria In Gujing-flavor Liquor Pit Mud Using Culture Independent Method |
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| Tang Bin,Liu Jin-ying,Zhou Qing-qu,Li An-jun,Wan Chun-huan,Tang You-hong.Phylogenetic Diversity Analysis of Bacteria In Gujing-flavor Liquor Pit Mud Using Culture Independent Method[J].Food and Fermentation Industries,2012(7):43-47. |
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| Authors: | Tang Bin Liu Jin-ying Zhou Qing-qu Li An-jun Wan Chun-huan Tang You-hong |
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| Affiliation: | 1(Anhui Polytechnic University,Engineering Technology Research Center of Anhui Microbial Fermentation,Wuhu 241000,China) 2(Anhui Gujing Distillery Company Limited,Haozhou 236826,China) |
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| Abstract: | Culture independent method was used to study the diversity of Pit Mud bacteria.The techniques included culture independent approach,PCR technique and 16S rDNA sequence homology analysis etc.The total DNA directly extracted from Pit Mud was used as PCR template.Random clones containing almost full size 16S rDNA sequences(about 1.5 kb) were sequenced and subjected to an on line similarity search.According to the sequences alignment and homology analysis,the bacteria of Pit Mud were consisted of Uncultured bacterium,Clostridium,Lactobacillus,Eubacterium,and Syntrophomonas. |
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| Keywords: | Gujing-flavor Liquor Pit Mud microbiology diversity culture independent approach phylogenetic analysis |
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