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| 高效液相色谱法测定柑橘中的柠檬苦素类似物 | |
| 引用本文： | 刘亮,戚向阳,董绪燕,李蕙蕙,刘传菊.高效液相色谱法测定柑橘中的柠檬苦素类似物[J].食品与发酵工业,2007,33(4):130-133. |
| 作者姓名： | 刘亮 戚向阳 董绪燕 李蕙蕙 刘传菊 |
| 作者单位： | 1. 武汉工业学院食品科技学院,湖北武汉,430023;华中农业大学食品科技学院,湖北武汉,430070 2. 华中农业大学食品科技学院,湖北武汉,430070 3. 中国农业科学院油料作物研究所,湖北武汉,430062 |
| 摘 要： | 建立了同时测定柑橘中柠檬苦素和诺米林2种苷元的高效液相色谱方法。色谱柱为Phenomenex C18(250mm×4.6mm,5μm),流动相为体积分数45%乙腈,流速为1.0mL/min,柱温为25℃,检测波长在210nm。经考察该方法可行性强,柠檬苦素和诺米林的平均回收率分别99.91%和98.28%,RSD分别为2.55%和2.30%。因此用该法测定了柑橘中的柠檬苦素和诺米林的含量,并取得了理想的结果。 |
| 关 键 词： | 柑橘 高效液相色谱法 柠檬苦素 诺米林 |
| 修稿时间： | 01 11 2007 12:00AM |
Determination of Limonoids in Citrus by High Performance Liquid Chromatography |
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| Liu Liang,Qi Xiangyang,Dong Xuyan,Li Huihui,Liu Chuanju.Determination of Limonoids in Citrus by High Performance Liquid Chromatography[J].Food and Fermentation Industries,2007,33(4):130-133. | |
| Authors: | Liu Liang Qi Xiangyang Dong Xuyan Li Huihui Liu Chuanju |
| Affiliation: | College of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023,China;College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;Institute of Oil Crops Research Chinese Academy of Agriculture Sciences, Wuhan, 430062, China |
| Abstract: | High performance liquid chromatography method for the simultaneous determination of two limonoid aglycosides,limonin and nomilin was established.Limonin and nomilin were separated by Phenomenex C18(250×4.6mm,5μm)column with acetonitrile-water,45∶55(V/V)as mobile phase and flow rate was 1mL/min.The column temperature was controlled at 25℃ and the wavelength of detector was set at 210nm.After exploration,the method was feasible,average recovery ratio of limonin and nomilin was 99.91%和98.28% respectively,RSD was 2.55% and 2.30%respectively.Thus,the content of limonin and nomilin in different citrus were determined,and the satisfied results were obtained. |
| Keywords: | citrus HPLC Limonin Nomilin |
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