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| miR-29b对肝癌细胞中甲基化转移酶表达的调控作用 | |
| 引用本文： | 吕赛群,金华君,钱其军.miR-29b对肝癌细胞中甲基化转移酶表达的调控作用[J].浙江理工大学学报,2011,28(1). |
| 作者姓名： | 吕赛群 金华君 钱其军 |
| 作者单位： | 1. 浙江理工大学生命科学学院,杭州,310018 2. 第二军医大学东方肝胆外科医院,上海,200438 3. 浙江理工大学生命科学学院,杭州,310018;第二军医大学东方肝胆外科医院,上海,200438 |
| 基金项目： | 国家自然科学基金(30730104) |
| 摘 要： | DNA甲基化异常在肿瘤发生中起着极为重要的作用,而基因组DNA的甲基化主要通过DNA甲基化转移酶(DNA methyhransferases,DNMTs)来完成。microRNA和甲基化转移酶的表达具有一定的联系。构建携带有miR-29b的腺病毒载体并包装病毒,鉴定正确后,将病毒感染肝癌细胞PLC,用qRT-PCR的方法检测miR-29b在PLC细胞中对甲基化转移酶表达的调控作用。结果表明,成功构建携带有miR-29b的腺病毒,qRT-PCR方法检测表明miR-29b能有效地调控甲基化转移酶的表达。 |
| 关 键 词： | miR-29b 甲基化转移酶 DNMT3A DNMT3B |
Regulating the Expression of DNA Methyltransferase in Liver Cancer Cells by miR-29b |
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| LU Sai-qun,JIN Hua-jun,QIAN Qi-jun.Regulating the Expression of DNA Methyltransferase in Liver Cancer Cells by miR-29b[J].Journal of Zhejiang Sci-tech University,2011,28(1). | |
| Authors: | LU Sai-qun JIN Hua-jun QIAN Qi-jun |
| Affiliation: | Lü Sai-qun,JIN Hua-jun,QIAN Qi-jun(1.School of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China,2.Eastern Hepatobiliary Surgical Hospital,Second Military Medical University,Shanghai 200438,China) |
| Abstract: | Abnormal DNA methylation plays an extremely important role in tumors.DNA methylation is completed through DNA methyltransferases(DNA methyhransferases,DMNTs).The miR-29 had a certain relationship with the expression of methylation transferase.In this study,an adenovirus vector carrying miR-29b is constructed and the virus is used to infect the liver cancer cell PLC.Then qRT-PCR is exploited to detect the relative quantity of miR-29b and methyltransferase in the infected PLC cells.The results show that the a... |
| Keywords: | miR-29b DNA methyhransferases DNMT3A DNMT3B |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |