| 静电纺丝素-明胶管状支架的结构与性能 |
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| 引用本文： | 王曙东,张幼珠,王红卫,尹桂波,董智慧,符伟国. 静电纺丝素-明胶管状支架的结构与性能[J]. 丝绸, 2009, 0(7) |
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| 作者姓名： | 王曙东 张幼珠 王红卫 尹桂波 董智慧 符伟国 |
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| 作者单位： | 1. 苏州大学,纺织与服装工程学院,江苏,苏州,215021
2. 复旦大学,附属中山医院,上海,200032 |
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| 基金项目： | 江苏省商校重点实验室开放研究课题 |
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| 摘 要： | 为研制组织工程小口径血管,以良好生物相容性的丝素蛋白、明胶为原料,通过静电纺丝法,以高速旋转的滚轴为收集装置,构建了丝素-明胶管状支架(直径为4.5 mm).测定其形貌结构、孔隙率和溶失率,并在该支架上进行人脐静脉内皮细胞(HUVECs)培养实验.结果表明:在缝素-明胶质量比例为70:30、纺丝液质量分数为13%、滚轴转速为1 000 r/min的条件下静电纺丝,可获得纤维直径较细、纤维分布较均匀、具有较高孔隙率的丝素-明胶管状支架:随着纺丝液质量分数的提高,丝素-明胶管状史架的溶失率降低,乙醇处理后管状支架溶失率大大降低;MTT显示细胞可以在支架上生长、增殖.
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| 关 键 词： | 静电纺丝 丝素 明胶 管状支架 支架结构 孔隙率 溶失率 细胞培养 |
Structure and Properties of Electro-spun Silk Fibroin-Gelatin Tubular Scaffold |
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| WANG Shu-dong,ZHANG You-zhu,WANG Hong-wei,YIN Gui-bo,DONG Zhi-hui,FU Wei-guo. Structure and Properties of Electro-spun Silk Fibroin-Gelatin Tubular Scaffold[J]. Silk Monthly, 2009, 0(7) |
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| Authors: | WANG Shu-dong ZHANG You-zhu WANG Hong-wei YIN Gui-bo DONG Zhi-hui FU Wei-guo |
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| Affiliation: | 1.College of Textile and Clothing Engineering;Soochow University;Suzhou 215021;China;2.Zhongshan Hospital;Fudan University;Shanghai 200032;China |
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| Abstract: | To develop a small-diameter tissue engineering blood vessel, the biocompatible silk fibroin(SF) and gelatin were used as materials, SF-gelatin tubular scaffold with 4.5 mm diameter was fabricated via electro-spinning, which was performed on a high speed rotating mandrel. The morphology, porosity and water-dissolved rate of the SF-gelatin were measured, the HUVECs were seed on the scaffold. The results showed that the SF- gelatin tubular scaffolds could be obtained which have small diameter fibers, well-prop... |
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| Keywords: | Electro-spinning Silk fibroin Gelatin Tubular scaffold Morphology Porosity Water-dissolved rate Cell culture |
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