|
|||||
|
|
| 发菜多糖的提取、纯化鉴定及理化特性研究 | |
| 引用本文： | 陈雪峰,李一当,贾士儒.发菜多糖的提取、纯化鉴定及理化特性研究[J].食品与发酵工业,2008,34(10). |
| 作者姓名： | 陈雪峰 李一当 贾士儒 |
| 作者单位： | 1. 天津科技大学生物工程学院,天津,300222;陕西科技大学生命科学与工程学院,陕西,西安,710021 2. 陕西科技大学生命科学与工程学院,陕西,西安,710021 3. 天津科技大学生物工程学院,天津,300222 |
| 基金项目： | 国家自然科学基金，天津市科技发展计划重点基金 |
| 摘 要： | 对发菜胞外多糖和发菜荚膜多糖分别进行提取研究,获得了2种多糖的理想提取工艺。分别将胞外粗多糖和荚膜粗多糖采用Sevag法脱蛋白,透析去除小分子杂质,DEAE-52柱层析分离纯化后,前者得到1种酸性糖(EPS),后者得到1种中性糖和2种酸性糖(CPSA、CPSB和CPSC),最后通过Sephadex G-100柱层析和紫外扫描检测,EPS、CPSA和CPSB均为单一多糖。 |
| 关 键 词： | 发菜多糖 提取 纯化 |
Study on Extraction,Purification Identification and Physical and Chemical Characteristics Assay of Nostoc flagelliforme Polysaccharides |
|
| Chen Xuefeng,Lu Yidang,Jia Shiru.Study on Extraction,Purification Identification and Physical and Chemical Characteristics Assay of Nostoc flagelliforme Polysaccharides[J].Food and Fermentation Industries,2008,34(10). | |
| Authors: | Chen Xuefeng Lu Yidang Jia Shiru |
| Affiliation: | Chen Xuefeng1,2,Lu Yidang2,Jia Shiru1 1 |
| Abstract: | The extraction of exopolysaccharide and capsularpolysaccharide from Nostoc flagelliforme was studied separately to obtain the optimal extraction technology of these two kinds of polysaccharides.The protein in raw polysaccharide was removed by Sevag and the small molecule was removed by dialysis.Through DEAE-52 cellulose column chromatography,EPS was obtained from exopolysaccharide,and CPSA,CPSB and CPSC were obtained from capsularpolysaccharide.EPS,CPSA and CPSB were identified as homogenous components by gel filtration chromatography(Sephadex G-100) and UV spectrum. |
| Keywords: | Nostoc flagelliforme polysaccharides extraction purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |