超声波预处理微波萃取葛根总黄酮的工艺优化

以湘西野葛根为原料，优化葛根总黄酮超声微波双辅助萃取工艺。研究超声提取温度、超声作用时间、微波功率、微波作用时间、固液比5个影响因素对葛根总黄酮提取量的影响，采用紫外分光光度计测定葛粉中总黄酮的含量，建立葛根总黄酮的最佳提取工艺条件。结果表明，在以80%的乙醇为提取溶剂、微波辐射功率300W、微波辐射时间9min、固液比1:25(g/mL)、超声提取温度50℃、超声提取时间30min的条件下，总黄酮的提取量可达14.685mg/g。

Process Optimization for Extraction of Total Flavonoids from Radix Pueraria by Ultrasonic Followed by Microwave Treatment

Ultrasonic followed by microwave treatment was used to establish a novel method to extract total flavonoids from Radix Pueraria.The effects of five extractions,namely temperature during ultrasonic treatment,ultrasonic treatment time,microwave power and treatment time and solid-to-liquid ratio on the extraction yield of total flavonoids from Radix Pueraria were explored.The quantification of total flavonoids was conducted using a UV spectrophotometer.The results showed that the maximum yield of total flavono...