响应面法优化丹参叶总酚酸超声波辅助提取工艺

对丹参叶总酚酸的超声提取工艺优化进行研究。在单因素试验基础上,选择提取溶剂乙醇体积分数、提取时间、温度和液料比为自变量,以丹参叶总酚酸得率为响应值,采用Box-Behnken试验设计方法,研究各自变量及其交互作用对丹参叶总酚酸提取率的影响。利用Design Expert软件得到回归方程的预测模型并进行响应面分析,确定超声波辅助提取丹参叶总酚酸的最佳条件为乙醇体积分数63.00%、浸提时间43.00min、温度50.00℃、液料比33:1(mL/g),在此条件下,总酚酸提取率达到7.78%。验证实验表明,所得模型方程能较好地预测实验结果。

Optimization of Ultrasound-assisted Extraction of Total Phenolic Acids from Salvia miltiorrhiza Bunge. Leaves Using Response Surface Analysis

Response surface methodology was used to optimize the ultrasound-assisted extraction of total phenolic acids from Salvia miltiorrhiza bunge.Leaves.The extraction efficiency of total phenolic acids was investigated with respect to four variables including ethanol concentration,time,temperature and liquid-to-solid ratio.On the basis of a series of one-factor-at-a-time experiments,a polynomial regression model equation was fitted by the combined use of Box-Behnken experimental design and regression analysis.By...