| 大孔吸附树脂分离纯化软枣猕猴桃总黄酮 |
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| 引用本文： | 刘长江,栾云峰,王菲,梁爽,杨智超.大孔吸附树脂分离纯化软枣猕猴桃总黄酮[J].食品科学,2011,32(12):145. |
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| 作者姓名： | 刘长江 栾云峰 王菲 梁爽 杨智超 |
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| 作者单位： | 沈阳农业大学食品学院; |
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| 基金项目： | 农业部公益性行业(农业)科研专项(200903013) |
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| 摘 要： | 以总黄酮吸附率、解吸率为考察指标,研究大孔吸附树脂分离纯化软枣猕猴桃总黄酮的工艺条件。结果表明:HPD600型树脂对软枣猕猴桃总黄酮有较好的吸附性能,在上样液质量浓度0.5～0.6mg/mL、pH3～4、吸附流速2mL/min、上样量3BV条件下,以3BV的水冲洗树脂柱、用4BV 80%乙醇溶液以2mL/min流速洗脱,处理后的软枣猕猴桃总黄酮平均回收率可达86.5%,平均纯度为37.2%。HPD600型大孔吸附树脂适合对软枣猕猴桃黄酮进行分离纯化。
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| 关 键 词： | 大孔吸附树脂 软枣猕猴桃 总黄酮 分离纯化 |
Macroporous Resin Adsorption for Purification of Total Flavonoids from Actinidia arguta Fruits |
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| LIU Chang-jiang,LUAN Yun-feng,WANG Fei,LIANG Shuang,YANG Zhi-Chao.Macroporous Resin Adsorption for Purification of Total Flavonoids from Actinidia arguta Fruits[J].Food Science,2011,32(12):145. |
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| Authors: | LIU Chang-jiang LUAN Yun-feng WANG Fei LIANG Shuang YANG Zhi-Chao |
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| Affiliation: | LIU Chang-jiang,LUAN Yun-feng,WANG Fei,LIANG Shuang,YANG Zhi-Chao (College of Food Science,Shenyang Agricultural University,Shenyang 110866,China) |
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| Abstract: | In the present study,macroporous resin adsorption was used to probe the purification of total flavonoids from the crude extract of Actinidia arguta fruits,prepared by ultrasonic-assisted ethanol and reextraction with petroleum ether.Among the nine macroporous resins investigated,HPD 600 exhibited the best performance in adsorbing and desorbing total flavonoids from Actinidia arguta fruits and its optimum working parameters were sample solution pH 3-4,sample concentration of 0.5 mg/mL,sample loading amount o... |
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| Keywords: | macroporous resin Actinidia arguta flavonoids purification |
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