| 大孔吸附树脂分离纯化洋葱皮黄酮的研究 |
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| 引用本文： | 徐怀德,陈佳,包蓉,刘坤,李晋.大孔吸附树脂分离纯化洋葱皮黄酮的研究[J].食品科学,2011,32(12):133. |
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| 作者姓名： | 徐怀德 陈佳 包蓉 刘坤 李晋 |
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| 作者单位： | 西北农林科技大学食品科学与工程学院;西北农林科技大学林学院; |
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| 摘 要： | 以黄酮含量为指标,比较6种大孔吸附树脂对洋葱皮黄酮的吸附和解吸效果。通过静态吸附与解吸实验,筛选出效果较好的X-5树脂进行动态实验研究。结果表明:X-5树脂纯化洋葱皮黄酮的工艺条件为吸附流速2mL/min、上样溶液pH5.0、上样液质量浓度0.5mg/mL、50mL 80%乙醇作为洗脱液,洗脱流速lmL/min。经X-5树脂纯化后,洋葱皮黄酮纯度从7.95%提高到80.78%。三次重结晶后其纯度可达94.5%。该方法简单可行,纯化效果好,适合于工业化生产。
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| 关 键 词： | 大孔吸附树脂 分离 纯化 洋葱皮 黄酮 |
Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption |
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| XU Huai-de,CHEN Jia,BAO Rong,LIU Kun,LI Jin.Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption[J].Food Science,2011,32(12):133. |
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| Authors: | XU Huai-de CHEN Jia BAO Rong LIU Kun LI Jin |
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| Affiliation: | XU Huai-de1,CHEN Jia1,BAO Rong2,LIU Kun1,LI Jin1 (1.College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China,2.College of Forestry,China) |
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| Abstract: | The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared.Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel.The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL,sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min.After the purification,the p... |
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| Keywords: | macroporous adsorption resin separation purification onion peel flavonoids |
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