| SM_2单克隆抗体的初步应用研究 |
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| 引用本文： | 丁良君,李继昌,付锐,李德山.SM_2单克隆抗体的初步应用研究[J].食品工业,2011(7). |
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| 作者姓名： | 丁良君 李继昌 付锐 李德山 |
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| 作者单位： | 东北农业大学博士后流动站;东北农业大学生命科学院;东北农业大学动物医学院;伊春市金山屯区人民医院; |
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| 基金项目： | 哈尔滨市科技创新人才研究专项资金,项目编号:2010RFQXN109; 国家自然科学基金(青年科学基金项目),项目批准号:31001084 |
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| 摘 要： | 以磺胺二甲嘧啶(SM2)-人血清白蛋白(HSA)为免疫抗原和SM2-卵清白蛋白(OVA)为包被抗原。制备SM2单克隆抗体;利用杂交瘤技术和有限稀释法经亚克隆,得到三株特异性稳定分泌SM2抗体的杂交瘤细胞,与其他四种磺胺药和两种载体蛋白HSA、OVA均无交叉反应。利用本试验制备的单克隆抗体初步建立了动物组织中检测SM2的间接竞争ELISA方法。最低检出限为9.95 ng/mL,灵敏度为47.12 ng/mL。
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| 关 键 词： | 磺胺二甲嘧啶 单克隆抗体 酶联免疫吸附试验 |
The Initial Utilized of Monoclonal Antibody of Sulfamethazine |
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| Ding Liang-jun,Li Ji-chang,Fu Rui,Li De-shan .Northeast Agricultural University Postdoctoral Station,.College of Life Sciences,Northeast Agricultural University,.College of Veterinary Medicine,North east Agricultural University.The Initial Utilized of Monoclonal Antibody of Sulfamethazine[J].The Food Industry,2011(7). |
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| Authors: | Ding Liang-jun Li Ji-chang Fu Rui Li De-shan Northeast Agricultural University Postdoctoral Station College of Life Sciences Northeast Agricultural University College of Veterinary Medicine North east Agricultural University |
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| Affiliation: | Ding Liang-jun1,2,Li Ji-chang3,Fu Rui4,Li De-shan2 1.Northeast Agricultural University Postdoctoral Station(Harbin 150030),2.College of Life Sciences,Northeast Agricultural University(Harbin 150030),3.College of Veterinary Medicine,North east Agricultural University(Harbin 150030),4.Forestry Hospital of Jin Shan Tun of Yi Chun(Yichun 150026) |
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| Abstract: | Sulfamethazine-human serum albumin was used for the immunogen,and SM2-ovalbumin was used for the coating antigen,to make sulfamethazine monoclonal antibody.Using cell fusion and limiting dilution method to reclone to get three hybridoma strains,which could stable secret monoclonal antibody.No cross-reactivity was seen between the McAb and the other four sulfonamides,as well as the two carrier proteins.The monoclonal antibody was used to developed an indirect competitive inhibition enzyme linked immune absor... |
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| Keywords: | sulfamethazine monoclonal antibody ELISA |
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