| 米曲霉菌种选育中原生质体制备方法的研究 |
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| 引用本文： | 宋鹏,陈亮,李德霞.米曲霉菌种选育中原生质体制备方法的研究[J].食品工业,2011(7). |
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| 作者姓名： | 宋鹏 陈亮 李德霞 |
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| 作者单位： | 河南科技大学农学院;河南工业大学生物工程学院; |
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| 基金项目： | 河南科技大学实验技术开发基金项目(SY0809045); 河南科技大学SRTP项目(2010082) |
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| 摘 要： | 米曲霉(Aspergillus oryzae)是一种好气性真菌,在生长过程中可以产生多种酶,广泛的应用于食品发酵工业中。因此,米曲霉优良菌株的选育有着重要意义和实用价值。围绕原生质体制备的方法,重点阐述了菌丝和孢子制备原生质体的优缺点以及破壁酶的选择,为米曲霉菌种选育提供理论支持和技术帮助。
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| 关 键 词： | 米曲霉 原生质体 制备 孢子 酶制剂 |
Research on the Protoplast Preparation Methods of Aspergillus oryzae in Strain Breeding |
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| Song Peng,Chen Liang,Li De-xia .College of Agriculture,Henan University of Science , Technology .School of Bioengineering,Henan University of Technology.Research on the Protoplast Preparation Methods of Aspergillus oryzae in Strain Breeding[J].The Food Industry,2011(7). |
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| Authors: | Song Peng Chen Liang Li De-xia College of Agriculture Henan University of Science Technology School of Bioengineering Henan University of Technology |
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| Affiliation: | Song Peng1,Chen Liang2,Li De-xia1 1.College of Agriculture,Henan University of Science and Technology(Luoyang 471003) 2.School of Bioengineering,Henan University of Technology(Zhengzhou 450001) |
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| Abstract: | Aspergillus oryzae is an aerobic fungus,which plays an important role in the industry.Aspergillus oryzae can produce many valuable enzymes applied to the food industry widely.So it is of great significance to select fine strain.Based on the technology of the protoplast preparation,advantage and disadvantage of different methods from hypha and spore and selection of zymolyases are described reasonedly and intensively,which can provide theory and technique help for the strain breeding of aspergillus oryzae. |
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| Keywords: | aspergillus oryzae protoplast preparation spore enzyme preparation |
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