| 大孔树脂纯化革皮氏海参总皂苷工艺 |
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| 引用本文： | 于林芳,王超,王玉明,王林,董平,薛勇,薛长湖.大孔树脂纯化革皮氏海参总皂苷工艺[J].食品科学,2011,32(12):1. |
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| 作者姓名： | 于林芳 王超 王玉明 王林 董平 薛勇 薛长湖 |
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| 作者单位： | 中国海洋大学食品科学与工程学院; |
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| 基金项目： | 国家自然科学青年基金项目(31000795); 国际科技合作项目(2010DFA31330) |
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| 摘 要： | 比较7种大孔吸附树脂对革皮氏海参总皂苷的静态吸附和解吸效果,从中筛选出适合该海参总皂苷分离纯化的树脂,并对其动态吸附和解吸性能进行研究。结果表明,XAD761树脂最适合革皮氏海参总皂苷的纯化。当上样液质量浓度2mg/mL,流速0.8mL/min,上样液体积9BV时,树脂达到动态吸附饱和。再用5BV 70%乙醇,以1mL/min的流速可以洗脱完全,解吸率为93.31%。经过树脂的纯化,皂苷的纯度由原来的21%提高到65%。
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| 关 键 词： | 海参 皂苷 分离纯化 大孔树脂 |
Macroporous Resin Purification of Saponins from the sea cucumber Pearsonothria graeffei |
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| YU Lin-fang,WANG Chao,WANG Yu-ming,WANG Lin,DONG Ping,XUE Yong,XUE Chang-hu.Macroporous Resin Purification of Saponins from the sea cucumber Pearsonothria graeffei[J].Food Science,2011,32(12):1. |
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| Authors: | YU Lin-fang WANG Chao WANG Yu-ming WANG Lin DONG Ping XUE Yong XUE Chang-hu |
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| Affiliation: | YU Lin-fang,WANG Chao,WANG Yu-ming,WANG Lin,DONG Ping,XUE Yong,XUE Chang-hu(College of Food Science and Technology,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | The adsorption and desorption rates of 7 types of macroporous resins towards total saponins from the sea cucumber Pearsonothria graeffei were investigated.Among the studied macroporous resins,XAD761 resin was the most suitable to purify crude total flavonoid extract from Pearsonothria graeffei.When 9 BV of 2 mg/mL sample was flowed through XAD761 resin column at a speed of 0.8 mL/min,the adsorption reached dynamic equilibrium.The adsorbed saponins could be completely eluted with 5 BV of 70%(V/V) ethanol at ... |
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| Keywords: | sea cucumber saponin purification macroporous resin |
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