| 九华山黄精多糖的分离纯化及化学表征 |
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| 引用本文： | 张庭廷,胡威,汪好芬,冉颖霞,郑春艳. 九华山黄精多糖的分离纯化及化学表征[J]. 食品科学, 2011, 32(10): 48-51. DOI: 10.7506/spkx1002-6630-201110011 |
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| 作者姓名： | 张庭廷 胡威 汪好芬 冉颖霞 郑春艳 |
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| 作者单位： | 安徽师范大学生命科学学院，生物环境与生态安全安徽省高校重点实验室 |
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| 基金项目： | 芜湖市科技开发重点项目(200761); 安徽师范大学重点项目(200918) |
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| 摘 要： | 采用传统方法、酶法、超声波提取以及酶法辅助超声等多种手段对九华山多花黄精多糖进行提取，并用DEAE-纤维素柱层析与交联葡聚糖分子筛法等对多糖进行纯化、分级以及相对分子质量测定，采用红外光谱以及气相色谱法等对多糖的构型和组成进行分析。结果表明：纤维素酶辅以超声提取方法最佳，多糖得率最高；多糖为杂多糖，其相对分子质量为8912，其组成为果糖:葡萄糖=8.7:1。
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| 关 键 词： | 黄精多糖 分离纯化 化学表征 |
Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain |
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| ZHANG Ting-ting,HU Wei,WANG Hao-fen,RAN Ying-xia,ZHENG Chun-yan. Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain[J]. Food Science, 2011, 32(10): 48-51. DOI: 10.7506/spkx1002-6630-201110011 |
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| Authors: | ZHANG Ting-ting HU Wei WANG Hao-fen RAN Ying-xia ZHENG Chun-yan |
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| Affiliation: | Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, College of Life Sciences,Anhui Normal University, Wuhu 241000, China |
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| Abstract: | The conventional method,enzymolysis,ultrasonic extraction and ultrasonic-assisted enzymolysis were compared for their effectiveness in extracting polysaccharides from Polygonatum cyrtonema Hua in Jiuhua Mountain.Ultrasonic-assisted enzymolysis was evaluated as the best method due to the highest polysaccharide yield.The polysaccharide extract obtained by the selected method was a single polysaccharide component according to DEAE-cellulose column chromatographic analysis.Infrared spectral analysis revealed th... |
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| Keywords: | polysaccharides from Polygonatum cyrtonema Hua separation and purification chemical characterization |
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