| 牛乳铁蛋白LF单克隆抗体的制备 |
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| 引用本文： | 韩秀娥,张萍,张炳丽,王雪峰,相文华. 牛乳铁蛋白LF单克隆抗体的制备[J]. 食品工业, 2011, 0(8) |
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| 作者姓名： | 韩秀娥 张萍 张炳丽 王雪峰 相文华 |
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| 作者单位： | 东北农业大学黑龙江省乳品工业技术开发中心;东北农业大学动物医学院;哈尔滨市疾病预防控制中心;中国农业科学院哈尔滨兽医研究所; |
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| 基金项目： | 黑龙江省博士后基金(编号为LBH-Z09257); 东北农业大学校启动基金 |
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| 摘 要： | 制备牛乳铁蛋白单克隆抗体,研究利用标准LF蛋白作为免疫原,免疫六周龄Balb/c雌性小鼠,运用淋巴细胞杂交瘤技术,通过间接ELISA筛选,采用有限稀释法,经过3次克隆筛选,获得两株稳定分泌抗LF蛋白的杂交瘤细胞株,命名为:L1,并进行了亚类鉴定。结果表明:该杂交瘤细胞培养上清抗体效价L1为1:4.56×104;腹水L1为1:5.21×106;L1分泌的单克隆抗体为IgG1型,轻链为λ链抗体。
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| 关 键 词： | LF 细胞培养 单克隆抗体 |
Preparation of Monoclonal Antibodies Against LF of Bovine |
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| Han Xiu-e,Zhang Ping,Zhang Bing-li,Wang Xue-feng,Xiang Wen-hua .Heilongjiang Dairy Industry Technical Development Center,Northeast Agricultural University,.College of Veterinary Medicine,.Harbin Center for Disease Control , Prevention,.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences. Preparation of Monoclonal Antibodies Against LF of Bovine[J]. The Food Industry, 2011, 0(8) |
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| Authors: | Han Xiu-e Zhang Ping Zhang Bing-li Wang Xue-feng Xiang Wen-hua .Heilongjiang Dairy Industry Technical Development Center Northeast Agricultural University .College of Veterinary Medicine .Harbin Center for Disease Control Prevention .National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences |
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| Affiliation: | Han Xiu-e1,Zhang Ping2,Zhang Bing-li3,Wang Xue-feng4,Xiang Wen-hua4 1.Heilongjiang Dairy Industry Technical Development Center,Northeast Agricultural University(Harbin 150001),2.College of Veterinary Medicine,3.Harbin Center for Disease Control and Prevention(Harbin 150001),4.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences(Harbin 150030) |
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| Abstract: | |
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| Keywords: | LF cells culture Monoclonal Antibody |
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